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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Human Mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), partial CSB-YP013439HU
CSB-EP013439HU
CSB-BP013439HU
CSB-MP013439HU
CSB-EP013439HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Chloride intracellular channel protein 3 (CLIC3), partial CSB-YP005548HU1
CSB-EP005548HU1
CSB-BP005548HU1
CSB-MP005548HU1
CSB-EP005548HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Guanine nucleotide-binding protein subunit alpha-14 (GNA14) CSB-YP009586HU
CSB-EP009586HU
CSB-BP009586HU
CSB-MP009586HU
CSB-EP009586HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Guanylyl cyclase-activating protein 3 (GUCA1C) CSB-YP010046HU
CSB-EP010046HU
CSB-BP010046HU
CSB-MP010046HU
CSB-EP010046HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Tetraspanin-13 (TSPAN13), partial CSB-YP025150HU1
CSB-EP025150HU1
CSB-BP025150HU1
CSB-MP025150HU1
CSB-EP025150HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human N (G),N (G)-dimethylarginine dimethylaminohydrolase 2 (DDAH2) CSB-YP006580HU
CSB-BP006580HU
CSB-MP006580HU
CSB-EP006580HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Uncharacterized protein C6orf47 (C6orf47) CSB-YP004126HU
CSB-EP004126HU
CSB-BP004126HU
CSB-MP004126HU
CSB-EP004126HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Disks large-associated protein 3 (DLGAP3), partial CSB-YP006942HU
CSB-EP006942HU
CSB-BP006942HU
CSB-MP006942HU
CSB-EP006942HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Probable tRNA pseudouridine synthase 2 (TRUB2) CSB-YP025106HU
CSB-EP025106HU
CSB-BP025106HU
CSB-MP025106HU
CSB-EP025106HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Probable tubulin polyglutamylase TTLL1 (TTLL1) CSB-YP025253HU
CSB-EP025253HU
CSB-BP025253HU
CSB-MP025253HU
CSB-EP025253HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Eomesodermin homolog (EOMES) CSB-YP007701HU
CSB-EP007701HU
CSB-BP007701HU
CSB-MP007701HU
CSB-EP007701HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Integrin beta-like protein 1 (ITGBL1) CSB-YP011893HU
CSB-BP011893HU
CSB-EP011893HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Immunoglobulin superfamily member 6 (IGSF6), partial CSB-YP011564HU1
CSB-EP011564HU1
CSB-BP011564HU1
CSB-MP011564HU1
CSB-EP011564HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Reversion-inducing cysteine-rich protein with Kazal motifs (RECK), partial CSB-YP019536HU
CSB-EP019536HU
CSB-BP019536HU
CSB-MP019536HU
CSB-EP019536HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Actin-associated protein FAM107A (FAM107A) CSB-YP007987HU
CSB-EP007987HU
CSB-BP007987HU
CSB-MP007987HU
CSB-EP007987HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Adenomatous polyposis coli protein 2 (APC2), partial CSB-YP001895HU
CSB-EP001895HU
CSB-BP001895HU
CSB-MP001895HU
CSB-EP001895HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human B-cell lymphoma/leukemia 10 (BCL10) CSB-YP002608HU
CSB-BP002608HU
CSB-MP002608HU
CSB-EP002608HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Cleft lip and palate transmembrane protein 1 (CLPTM1), partial CSB-YP005580HU1
CSB-EP005580HU1
CSB-BP005580HU1
CSB-MP005580HU1
CSB-EP005580HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Serine/threonine-protein kinase PAK 4 (PAK4) CSB-YP017408HU
CSB-BP017408HU
CSB-MP017408HU
CSB-EP017408HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Amyloid beta A4 precursor protein-binding family A member 3 (APBA3) CSB-YP001889HU
CSB-EP001889HU
CSB-BP001889HU
CSB-MP001889HU
CSB-EP001889HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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