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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Human Glycosaminoglycan xylosylkinase (FAM20B), partial CSB-YP008173HU1
CSB-EP008173HU1
CSB-BP008173HU1
CSB-MP008173HU1
CSB-EP008173HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Low-density lipoprotein receptor-related protein 3 (LRP3), partial CSB-YP013098HU1
CSB-EP013098HU1
CSB-BP013098HU1
CSB-MP013098HU1
CSB-EP013098HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Slit homolog 1 protein (SLIT1), partial CSB-YP021767HU
CSB-EP021767HU
CSB-BP021767HU
CSB-MP021767HU
CSB-EP021767HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human LIM domain-binding protein 3 (LDB3) CSB-YP012831HU
CSB-BP012831HU
CSB-MP012831HU
CSB-EP012831HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Astrotactin-2 (ASTN2), partial CSB-YP002252HU
CSB-EP002252HU
CSB-BP002252HU
CSB-MP002252HU
CSB-EP002252HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Obscurin-like protein 1 (OBSL1), partial CSB-YP016254HU
CSB-EP016254HU
CSB-BP016254HU
CSB-MP016254HU
CSB-EP016254HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Suppressor of cytokine signaling 5 (SOCS5) CSB-YP022394HU
CSB-EP022394HU
CSB-BP022394HU
CSB-MP022394HU
CSB-EP022394HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Phosphatase and actin regulator 2 (PHACTR2) CSB-YP017881HU
CSB-EP017881HU
CSB-BP017881HU
CSB-MP017881HU
CSB-EP017881HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Xylulose kinase (XYLB) CSB-YP026240HU
CSB-EP026240HU
CSB-BP026240HU
CSB-MP026240HU
CSB-EP026240HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial (NDUFS7) CSB-YP015666HU
CSB-EP015666HU
CSB-BP015666HU
CSB-MP015666HU
CSB-EP015666HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Cadherin-16 (CDH16), partial CSB-YP005041HU
CSB-EP005041HU
CSB-BP005041HU
CSB-MP005041HU
CSB-EP005041HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Protein NipSnap homolog 2 (GBAS) CSB-YP009293HU
CSB-EP009293HU
CSB-BP009293HU
CSB-MP009293HU
CSB-EP009293HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human T-box transcription factor TBX10 (TBX10) CSB-YP023246HU
CSB-EP023246HU
CSB-BP023246HU
CSB-MP023246HU
CSB-EP023246HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Guanylate cyclase soluble subunit beta-2 (GUCY1B2) CSB-YP010051HU
CSB-EP010051HU
CSB-BP010051HU
CSB-MP010051HU
CSB-EP010051HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Vacuolar protein sorting-associated protein 4B (VPS4B) CSB-YP025915HU
CSB-EP025915HU
CSB-BP025915HU
CSB-MP025915HU
CSB-EP025915HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ectonucleoside triphosphate diphosphohydrolase 3 (ENTPD3), partial CSB-YP007692HU
CSB-EP007692HU
CSB-BP007692HU
CSB-MP007692HU
CSB-EP007692HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Pituitary homeobox 3 (PITX3) CSB-YP018044HU
CSB-EP018044HU
CSB-BP018044HU
CSB-MP018044HU
CSB-EP018044HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Nuclear receptor corepressor 1 (NCOR1), partial CSB-YP015547HU
CSB-BP015547HU
CSB-MP015547HU
CSB-EP015547HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Serine/threonine-protein kinase ULK1 (ULK1), partial CSB-YP025612HU
CSB-EP025612HU
CSB-BP025612HU
CSB-MP025612HU
CSB-EP025612HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Vesicle-trafficking protein SEC22b (SEC22B), partial CSB-YP020945HU1
CSB-EP020945HU1
CSB-BP020945HU1
CSB-MP020945HU1
CSB-EP020945HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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