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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Human Katanin p60 ATPase-containing subunit A1 (KATNA1) CSB-YP011990HU
CSB-EP011990HU
CSB-BP011990HU
CSB-MP011990HU
CSB-EP011990HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Cytokine receptor-like factor 1 (CRLF1) CSB-YP005982HU
CSB-MP005982HU
CSB-EP005982HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human PC4 and SFRS1-interacting protein (PSIP1) CSB-YP018860HU
CSB-BP018860HU
CSB-MP018860HU
CSB-EP018860HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Ceroid-lipofuscinosis neuronal protein 5 (CLN5) CSB-YP005567HU
CSB-BP005567HU
CSB-MP005567HU
CSB-EP005567HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human KH domain-containing, RNA-binding, signal transduction-associated protein 3 (KHDRBS3) CSB-YP012156HU
CSB-EP012156HU
CSB-BP012156HU
CSB-MP012156HU
CSB-EP012156HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Splicing factor 3B subunit 1 (SF3B1), partial CSB-YP021125HU
CSB-EP021125HU
CSB-BP021125HU
CSB-MP021125HU
CSB-EP021125HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human WW domain-binding protein 4 (WBP4) CSB-YP025978HU
CSB-EP025978HU
CSB-BP025978HU
CSB-MP025978HU
CSB-EP025978HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Interferon-inducible double stranded RNA-dependent protein kinase activator A (PRKRA) CSB-YP018717HU
CSB-EP018717HU
CSB-BP018717HU
CSB-MP018717HU
CSB-EP018717HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Mediator of RNA polymerase II transcription subunit 6 (MED6) CSB-YP013666HU
CSB-EP013666HU
CSB-BP013666HU
CSB-MP013666HU
CSB-EP013666HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial (GCAT) CSB-YP009308HU
CSB-EP009308HU
CSB-BP009308HU
CSB-MP009308HU
CSB-EP009308HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Left-right determination factor 1 (LEFTY1) CSB-YP012857HU
CSB-EP012857HU
CSB-BP012857HU
CSB-MP012857HU
CSB-EP012857HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Uroplakin-3a (UPK3A), partial CSB-YP025657HU1
CSB-EP025657HU1
CSB-BP025657HU1
CSB-MP025657HU1
CSB-EP025657HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human U5 small nuclear ribonucleoprotein 200 kDa helicase (SNRNP200), partial CSB-YP022319HU
CSB-EP022319HU
CSB-BP022319HU
CSB-MP022319HU
CSB-EP022319HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Surfeit locus protein 6 (SURF6) CSB-YP022966HU
CSB-EP022966HU
CSB-BP022966HU
CSB-MP022966HU
CSB-EP022966HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Serine/threonine-protein kinase 16 (STK16) CSB-YP022835HU
CSB-BP022835HU
CSB-MP022835HU
CSB-EP022835HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Transient receptor potential cation channel subfamily A member 1 (TRPA1), partial CSB-YP025074HU
CSB-BP025074HU
CSB-MP025074HU
CSB-EP025074HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Rhomboid-related protein 1 (RHBDL1), partial CSB-YP019671HU1
CSB-EP019671HU1
CSB-BP019671HU1
CSB-MP019671HU1
CSB-EP019671HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Serpin I2 (SERPINI2) CSB-YP021090HU
CSB-EP021090HU
CSB-BP021090HU
CSB-MP021090HU
CSB-EP021090HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Uroplakin-1b (UPK1B), partial CSB-YP025655HU1
CSB-EP025655HU1
CSB-BP025655HU1
CSB-MP025655HU1
CSB-EP025655HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative hydrolase RBBP9 (RBBP9) CSB-YP019394HU
CSB-EP019394HU
CSB-BP019394HU
CSB-MP019394HU
CSB-EP019394HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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