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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mouse CDGSH iron-sulfur domain-containing protein 3, mitochondrial (Cisd3), partial CSB-YP005444MO
CSB-EP005444MO
CSB-BP005444MO
CSB-MP005444MO
CSB-EP005444MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Putative uncharacterized protein C1orf196 (C1orf196) CSB-YP003567HU
CSB-EP003567HU
CSB-BP003567HU
CSB-MP003567HU
CSB-EP003567HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Isthmin-1 (ISM1) CSB-YP011850HU
CSB-BP011850HU
CSB-MP011850HU
CSB-EP011850HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human 3-hydroxyacyl-CoA dehydratase 1 (PTPLA), partial CSB-YP019018HU1
CSB-EP019018HU1
CSB-BP019018HU1
CSB-MP019018HU1
CSB-EP019018HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Oligosaccharyltransferase complex subunit OSTC (Ostc), partial CSB-YP017264RA1
CSB-EP017264RA1
CSB-BP017264RA1
CSB-MP017264RA1
CSB-EP017264RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Solute carrier family 35 member G3 (Slc35g3), partial CSB-YP001648RA
CSB-EP001648RA
CSB-BP001648RA
CSB-MP001648RA
CSB-EP001648RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Glycolipid transfer protein (GLTP) CSB-YP009539RA
CSB-EP009539RA
CSB-BP009539RA
CSB-MP009539RA
CSB-EP009539RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Lysoplasmalogenase (Tmem86b), partial CSB-YP023889RA1
CSB-EP023889RA1
CSB-BP023889RA1
CSB-MP023889RA1
CSB-EP023889RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat SH3 domain-containing YSC84-like protein 1 (Sh3yl1) CSB-YP021246RA
CSB-EP021246RA
CSB-BP021246RA
CSB-MP021246RA
CSB-EP021246RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Prefoldin subunit 2 (Pfdn2) CSB-YP017813RA
CSB-EP017813RA
CSB-BP017813RA
CSB-MP017813RA
CSB-EP017813RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Orofacial cleft 1 candidate gene 1 protein homolog (OFCC1) CSB-YP016288CH
CSB-EP016288CH
CSB-BP016288CH
CSB-MP016288CH
CSB-EP016288CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Protein odd-skipped-related 1 (Osr1) CSB-YP017262RA
CSB-EP017262RA
CSB-BP017262RA
CSB-MP017262RA
CSB-EP017262RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat CDGSH iron-sulfur domain-containing protein 1 (Cisd1), partial CSB-YP005442RA1
CSB-EP005442RA1
CSB-BP005442RA1
CSB-MP005442RA1
CSB-EP005442RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Putative nuclease HARBI1 (Harbi1) CSB-YP010136RA
CSB-EP010136RA
CSB-BP010136RA
CSB-MP010136RA
CSB-EP010136RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Catenin beta-1 (CTNNB1) CSB-EP006169HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Ganglioside GM2 activator (GM2A) CSB-EP009565HUe0 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial (SDHA), partial CSB-EP020903HU1 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human ADP-ribosylation factor-like protein 2-binding protein (ARL2BP) CSB-EP897097HU 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human C-X-C motif chemokine 5 (CXCL5), partial CSB-EP006250HU1e0 1mg(1mg*1 or 500ug*2)/100ug/20ug
Recombinant Human Tumor necrosis factor ligand superfamily member 10 (TNFSF10), partial CSB-EP023985HUe0 1mg(1mg*1 or 500ug*2)/100ug/20ug

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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