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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Mouse Proline-rich transmembrane protein 2 (Prrt2), partial CSB-YP018805MO1
CSB-EP018805MO1
CSB-BP018805MO1
CSB-MP018805MO1
CSB-EP018805MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Maternal embryonic leucine zipper kinase (melk), partial CSB-YP013692DIL
CSB-EP013692DIL
CSB-BP013692DIL
CSB-MP013692DIL
CSB-EP013692DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum ADP-ribosylation factor 1 (arfA) CSB-YP001988DKK
CSB-EP001988DKK
CSB-BP001988DKK
CSB-MP001988DKK
CSB-EP001988DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharolobus solfataricus 3-hydroxy-3-methylglutaryl-coenzyme A reductase (hmgA) CSB-YP010565FPM
CSB-EP010565FPM
CSB-BP010565FPM
CSB-MP010565FPM
CSB-EP010565FPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Telomerase reverse transcriptase (trt1), partial CSB-YP023391SXV
CSB-EP023391SXV
CSB-BP023391SXV
CSB-MP023391SXV
CSB-EP023391SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phaffia rhodozyma Glyceraldehyde-3-phosphate dehydrogenase (GPD) CSB-YP009232EUI
CSB-EP009232EUI
CSB-BP009232EUI
CSB-MP009232EUI
CSB-EP009232EUI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe 4-aminobutyrate aminotransferase (SPAC19D5.07) CSB-YP001032SXV
CSB-EP001032SXV
CSB-BP001032SXV
CSB-MP001032SXV
CSB-EP001032SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Eukaryotic translation initiation factor 2-alpha kinase 1 (hri1), partial CSB-YP007510SXV
CSB-EP007510SXV
CSB-BP007510SXV
CSB-MP007510SXV
CSB-EP007510SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caenorhabditis elegans Histone deacetylase 4 (hda-4), partial CSB-YP010240CXY
CSB-EP010240CXY
CSB-BP010240CXY
CSB-MP010240CXY
CSB-EP010240CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Choristoneura fumiferana granulovirus Granulin CSB-YP009939DTC
CSB-EP009939DTC
CSB-BP009939DTC
CSB-MP009939DTC
CSB-EP009939DTC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca mulatta Peroxisome proliferator-activated receptor gamma (PPARG), partial CSB-YP018424MOW
CSB-EP018424MOW
CSB-BP018424MOW
CSB-MP018424MOW
CSB-EP018424MOW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Catharanthus roseus ADP-ribosylation factor 1 (ARF1) CSB-YP001988DQN
CSB-EP001988DQN
CSB-BP001988DQN
CSB-MP001988DQN
CSB-EP001988DQN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR), partial CSB-YP010565ZAX1
CSB-EP010565ZAX1
CSB-BP010565ZAX1
CSB-MP010565ZAX1
CSB-EP010565ZAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Spermidine synthase (speE) CSB-YP022668HUV
CSB-EP022668HUV
CSB-BP022668HUV
CSB-MP022668HUV
CSB-EP022668HUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Archaeoglobus fulgidus Ornithine carbamoyltransferase (argF) CSB-YP017270DOC
CSB-EP017270DOC
CSB-BP017270DOC
CSB-MP017270DOC
CSB-EP017270DOC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Archaeoglobus fulgidus Argininosuccinate lyase (argH) CSB-YP002213DOC
CSB-EP002213DOC
CSB-BP002213DOC
CSB-MP002213DOC
CSB-EP002213DOC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Archaeoglobus fulgidus CTP synthase (pyrG), partial CSB-YP006176DOC
CSB-EP006176DOC
CSB-BP006176DOC
CSB-MP006176DOC
CSB-EP006176DOC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Butyrivibrio fibrisolvens Adenine phosphoribosyltransferase (apt) CSB-YP001954BXZ
CSB-EP001954BXZ
CSB-BP001954BXZ
CSB-MP001954BXZ
CSB-EP001954BXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti Histidine ammonia-lyase (hutH), partial CSB-YP010123RKU
CSB-EP010123RKU
CSB-BP010123RKU
CSB-MP010123RKU
CSB-EP010123RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus delbrueckii subsp. bulgaricus Glyceraldehyde-3-phosphate dehydrogenase (gap) CSB-YP009232LMM
CSB-BP009232LMM
CSB-MP009232LMM
CSB-EP009232LMM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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