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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Yersinia pseudotuberculosis serotype IB Uroporphyrinogen decarboxylase (hemE) CSB-YP025678YAW
CSB-EP025678YAW
CSB-BP025678YAW
CSB-MP025678YAW
CSB-EP025678YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Adenosine deaminase (add) CSB-YP001268YAW
CSB-EP001268YAW
CSB-BP001268YAW
CSB-MP001268YAW
CSB-EP001268YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus CTP synthase (pyrG), partial CSB-YP006176BWR
CSB-EP006176BWR
CSB-BP006176BWR
CSB-MP006176BWR
CSB-EP006176BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Adenine phosphoribosyltransferase (apt) CSB-YP001954BWR
CSB-EP001954BWR
CSB-BP001954BWR
CSB-MP001954BWR
CSB-EP001954BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Glutaminase (glsA) CSB-YP009528BWR
CSB-EP009528BWR
CSB-BP009528BWR
CSB-MP009528BWR
CSB-EP009528BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella abortus Histidine ammonia-lyase (hutH), partial CSB-YP010123BWR
CSB-EP010123BWR
CSB-BP010123BWR
CSB-MP010123BWR
CSB-EP010123BWR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica Adenine phosphoribosyltransferase (apt) CSB-YP001954FFF
CSB-EP001954FFF
CSB-BP001954FFF
CSB-MP001954FFF
CSB-EP001954FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Francisella tularensis subsp. mediasiatica Uracil-DNA glycosylase (ung) CSB-YP025641FFF
CSB-EP025641FFF
CSB-BP025641FFF
CSB-MP025641FFF
CSB-EP025641FFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xanthomonas oryzae pv. oryzae CTP synthase (pyrG), partial CSB-YP006176XBC
CSB-EP006176XBC
CSB-BP006176XBC
CSB-MP006176XBC
CSB-EP006176XBC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia phytofirmans Choline dehydrogenase (betA), partial CSB-YP005335BXU
CSB-EP005335BXU
CSB-BP005335BXU
CSB-MP005335BXU
CSB-EP005335BXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium botulinum Argininosuccinate lyase (argH) CSB-YP002213DUG
CSB-EP002213DUG
CSB-BP002213DUG
CSB-MP002213DUG
CSB-EP002213DUG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella boydii serotype 18 CTP synthase (pyrG), partial CSB-YP006176SYZ
CSB-EP006176SYZ
CSB-BP006176SYZ
CSB-MP006176SYZ
CSB-EP006176SYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori GTP cyclohydrolase 1 (folE) CSB-YP009317HUZ
CSB-EP009317HUZ
CSB-BP009317HUZ
CSB-MP009317HUZ
CSB-EP009317HUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia tasmaniensis Argininosuccinate lyase (argH) CSB-YP002213ENB
CSB-EP002213ENB
CSB-BP002213ENB
CSB-MP002213ENB
CSB-EP002213ENB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Choline O-acetyltransferase (chat), partial CSB-YP005314DIL
CSB-EP005314DIL
CSB-BP005314DIL
CSB-MP005314DIL
CSB-EP005314DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium phaeobacteroides CTP synthase (pyrG), partial CSB-YP006176DSS
CSB-EP006176DSS
CSB-BP006176DSS
CSB-MP006176DSS
CSB-EP006176DSS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Acetylserotonin O-methyltransferase (Asmt) CSB-YP002216RA
CSB-EP002216RA
CSB-BP002216RA
CSB-MP002216RA
CSB-EP002216RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Ornithine carbamoyltransferase (arcB) CSB-YP017270AXI
CSB-EP017270AXI
CSB-BP017270AXI
CSB-MP017270AXI
CSB-EP017270AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Actinobacillus pleuropneumoniae serotype 7 Uracil-DNA glycosylase (ung) CSB-YP025641AXI
CSB-EP025641AXI
CSB-BP025641AXI
CSB-MP025641AXI
CSB-EP025641AXI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila ananassae Insulin receptor substrate 1 (chico), partial CSB-YP011829DKY
CSB-EP011829DKY
CSB-BP011829DKY
CSB-MP011829DKY
CSB-EP011829DKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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