Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Helianthus annuus Glutathione peroxidase 1 (GPXHA-1) CSB-YP009866HUO
CSB-EP009866HUO
CSB-BP009866HUO
CSB-MP009866HUO
CSB-EP009866HUO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Malus domestica Defender against cell death 1 (DAD1), partial CSB-YP006487MPS1
CSB-EP006487MPS1
CSB-BP006487MPS1
CSB-MP006487MPS1
CSB-EP006487MPS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori 6-phosphogluconolactonase (pgl) CSB-YP017861HUV
CSB-EP017861HUV
CSB-BP017861HUV
CSB-MP017861HUV
CSB-EP017861HUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter thermautotrophicus Argininosuccinate lyase (argH) CSB-YP002213MSR
CSB-EP002213MSR
CSB-BP002213MSR
CSB-MP002213MSR
CSB-EP002213MSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanothermobacter thermautotrophicus Glyceraldehyde-3-phosphate dehydrogenase (gap) CSB-YP009232MSR
CSB-EP009232MSR
CSB-BP009232MSR
CSB-MP009232MSR
CSB-EP009232MSR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Archaeoglobus fulgidus Phosphoserine phosphatase (AF_2138) CSB-YP018938DOC
CSB-EP018938DOC
CSB-BP018938DOC
CSB-MP018938DOC
CSB-EP018938DOC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Archaeoglobus fulgidus Phosphoglycolate phosphatase (AF_0444) CSB-YP017873DOC
CSB-EP017873DOC
CSB-BP017873DOC
CSB-MP017873DOC
CSB-EP017873DOC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Adenine phosphoribosyltransferase (apt) CSB-YP001954BRJ
CSB-EP001954BRJ
CSB-BP001954BRJ
CSB-MP001954BRJ
CSB-EP001954BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Ornithine decarboxylase antizyme 1 (OAZ) CSB-YP016245CH
CSB-EP016245CH
CSB-BP016245CH
CSB-MP016245CH
CSB-EP016245CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus mykiss Glyceraldehyde-3-phosphate dehydrogenase (gapdh) CSB-YP009232OEI
CSB-EP009232OEI
CSB-BP009232OEI
CSB-MP009232OEI
CSB-EP009232OEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ictalurus punctatus Rhodopsin (rho), partial CSB-YP019681ILX1
CSB-EP019681ILX1
CSB-BP019681ILX1
CSB-MP019681ILX1
CSB-EP019681ILX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Batrachocottus nicolskii Rhodopsin (rho), partial CSB-YP019681BSK1
CSB-EP019681BSK1
CSB-BP019681BSK1
CSB-MP019681BSK1
CSB-EP019681BSK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Apolipoprotein A-I (apoa1) CSB-YP001913DIL
CSB-EP001913DIL
CSB-BP001913DIL
CSB-MP001913DIL
CSB-EP001913DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Taurulus bubalis Rhodopsin (rho), partial CSB-YP019681TLM1
CSB-EP019681TLM1
CSB-BP019681TLM1
CSB-MP019681TLM1
CSB-EP019681TLM1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Amino-terminal enhancer of split (aes) CSB-YP001405XBE
CSB-EP001405XBE
CSB-BP001405XBE
CSB-MP001405XBE
CSB-EP001405XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Transmembrane protein 17 (TMEM17), partial CSB-YP023750CH1
CSB-EP023750CH1
CSB-BP023750CH1
CSB-MP023750CH1
CSB-EP023750CH1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Meleagris gallopavo Myoglobin (MB) CSB-YP013529MQV
CSB-EP013529MQV
CSB-BP013529MQV
CSB-MP013529MQV
CSB-EP013529MQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gorilla gorilla gorilla Apolipoprotein A-I (APOA1) CSB-YP001913GGZ
CSB-EP001913GGZ
CSB-BP001913GGZ
CSB-MP001913GGZ
CSB-EP001913GGZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Activating signal cointegrator 1 complex subunit 3 (ascc3), partial CSB-YP002198DIL
CSB-EP002198DIL
CSB-BP002198DIL
CSB-MP002198DIL
CSB-EP002198DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Folylpolyglutamate synthase (MET7), partial CSB-YP008851SVI
CSB-EP008851SVI
CSB-BP008851SVI
CSB-MP008851SVI
CSB-EP008851SVI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>