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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Rhizobium etli Glutaminase (glsA) CSB-YP009528RKL
CSB-EP009528RKL
CSB-BP009528RKL
CSB-MP009528RKL
CSB-EP009528RKL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopseudomonas palustris Argininosuccinate lyase (argH) CSB-YP002213RLV
CSB-EP002213RLV
CSB-BP002213RLV
CSB-MP002213RLV
CSB-EP002213RLV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proteus mirabilis Exoribonuclease 2 (rnb), partial CSB-YP007787EYZ
CSB-EP007787EYZ
CSB-BP007787EYZ
CSB-MP007787EYZ
CSB-EP007787EYZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila virilis Molybdenum cofactor sulfurase (mal), partial CSB-YP014702DMP
CSB-EP014702DMP
CSB-BP014702DMP
CSB-MP014702DMP
CSB-EP014702DMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila willistoni Tryptophan 2,3-dioxygenase (v) CSB-YP023351DMQ
CSB-EP023351DMQ
CSB-BP023351DMQ
CSB-MP023351DMQ
CSB-EP023351DMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila yakuba Spastin (spas), partial CSB-YP022488DMR1
CSB-EP022488DMR1
CSB-BP022488DMR1
CSB-MP022488DMR1
CSB-EP022488DMR1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Adenine phosphoribosyltransferase (apt) CSB-YP001954ENT
CSB-EP001954ENT
CSB-BP001954ENT
CSB-MP001954ENT
CSB-EP001954ENT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria CTP synthase (pyrG), partial CSB-YP006176BXP
CSB-EP006176BXP
CSB-BP006176BXP
CSB-MP006176BXP
CSB-EP006176BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Tryptophan 2,3-dioxygenase (kynA) CSB-YP023351BXP
CSB-EP023351BXP
CSB-BP023351BXP
CSB-MP023351BXP
CSB-EP023351BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia ambifaria Choline dehydrogenase (betA), partial CSB-YP005335BXP
CSB-EP005335BXP
CSB-BP005335BXP
CSB-MP005335BXP
CSB-EP005335BXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methylobacterium populi Argininosuccinate lyase (argH) CSB-YP002213MTC
CSB-EP002213MTC
CSB-BP002213MTC
CSB-MP002213MTC
CSB-EP002213MTC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cupriavidus taiwanensis Adenine phosphoribosyltransferase (apt) CSB-YP001954DZS
CSB-EP001954DZS
CSB-BP001954DZS
CSB-MP001954DZS
CSB-EP001954DZS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium marinum Uroporphyrinogen decarboxylase (hemE) CSB-YP025678MVS
CSB-EP025678MVS
CSB-BP025678MVS
CSB-MP025678MVS
CSB-EP025678MVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter baumannii Uracil-DNA glycosylase (ung) CSB-YP025641AWO
CSB-EP025641AWO
CSB-BP025641AWO
CSB-MP025641AWO
CSB-EP025641AWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylella fastidiosa CTP synthase (pyrG), partial CSB-YP006176XBN
CSB-EP006176XBN
CSB-BP006176XBN
CSB-MP006176XBN
CSB-EP006176XBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB GTP cyclohydrolase 1 (folE) CSB-YP009317YAW
CSB-EP009317YAW
CSB-BP009317YAW
CSB-MP009317YAW
CSB-EP009317YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype IB Exoribonuclease 2 (rnb), partial CSB-YP007787YAW
CSB-EP007787YAW
CSB-BP007787YAW
CSB-MP007787YAW
CSB-EP007787YAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sheep Cyclin-dependent kinase 4 CSB-YP005065SH
CSB-EP005065SH
CSB-BP005065SH
CSB-MP005065SH
CSB-EP005065SH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Porphyromonas gingivalis GTP cyclohydrolase 1 (folE) CSB-YP009317EXZ
CSB-EP009317EXZ
CSB-BP009317EXZ
CSB-MP009317EXZ
CSB-EP009317EXZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Treponema pallidum subsp. pallidum CTP synthase (pyrG), partial CSB-YP006176TPS
CSB-EP006176TPS
CSB-BP006176TPS
CSB-MP006176TPS
CSB-EP006176TPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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