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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Borrelia burgdorferi 6-phosphogluconolactonase (pgl) CSB-YP017861BUD
CSB-EP017861BUD
CSB-BP017861BUD
CSB-MP017861BUD
CSB-EP017861BUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium tuberculosis Thymidine phosphorylase (deoA) CSB-YP025392MVZ
CSB-EP025392MVZ
CSB-BP025392MVZ
CSB-MP025392MVZ
CSB-EP025392MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crocodylus porosus Transthyretin (TTR) CSB-YP025270DXX
CSB-EP025270DXX
CSB-BP025270DXX
CSB-MP025270DXX
CSB-EP025270DXX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus horikoshii Mevalonate kinase (mvk) CSB-YP015247FHX
CSB-EP015247FHX
CSB-BP015247FHX
CSB-MP015247FHX
CSB-EP015247FHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus horikoshii 3-hydroxy-3-methylglutaryl-coenzyme A reductase (hmgA) CSB-YP010565FHX
CSB-EP010565FHX
CSB-BP010565FHX
CSB-MP010565FHX
CSB-EP010565FHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus horikoshii Glyceraldehyde-3-phosphate dehydrogenase (gap) CSB-YP009232FHX
CSB-EP009232FHX
CSB-BP009232FHX
CSB-MP009232FHX
CSB-EP009232FHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caenorhabditis elegans Dual oxidase 1 (bli-3), partial CSB-YP007228CXY
CSB-EP007228CXY
CSB-BP007228CXY
CSB-MP007228CXY
CSB-EP007228CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trichosurus vulpecula Prolactin (PRL) CSB-YP018724TQG
CSB-EP018724TQG
CSB-BP018724TQG
CSB-MP018724TQG
CSB-EP018724TQG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aquifex aeolicus Phosphoglycolate phosphatase (gph) CSB-YP017873DNV
CSB-EP017873DNV
CSB-BP017873DNV
CSB-MP017873DNV
CSB-EP017873DNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aquifex aeolicus Argininosuccinate lyase (argH) CSB-YP002213DNV
CSB-EP002213DNV
CSB-BP002213DNV
CSB-MP002213DNV
CSB-EP002213DNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptomyces coelicolor Uroporphyrinogen decarboxylase (hemE) CSB-YP025678FOB
CSB-EP025678FOB
CSB-BP025678FOB
CSB-MP025678FOB
CSB-EP025678FOB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Insulin (ins), partial CSB-YP011742DIL
CSB-EP011742DIL
CSB-BP011742DIL
CSB-MP011742DIL
CSB-EP011742DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leontopithecus chrysopygus Beta-2-microglobulin (B2M) CSB-YP002486LOM
CSB-EP002486LOM
CSB-BP002486LOM
CSB-MP002486LOM
CSB-EP002486LOM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pithecia irrorata Beta-2-microglobulin (B2M) CSB-YP002486EWF
CSB-EP002486EWF
CSB-BP002486EWF
CSB-MP002486EWF
CSB-EP002486EWF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Mitogen-activated protein kinase kinase 4 (MKK4) CSB-YP013413DOA
CSB-EP013413DOA
CSB-BP013413DOA
CSB-MP013413DOA
CSB-EP013413DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Hordeum vulgare 4-hydroxyphenylpyruvate dioxygenase CSB-YP010698HWQ
CSB-EP010698HWQ
CSB-BP010698HWQ
CSB-MP010698HWQ
CSB-EP010698HWQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi Uracil-DNA glycosylase (ung) CSB-YP025641BUD
CSB-EP025641BUD
CSB-BP025641BUD
CSB-MP025641BUD
CSB-EP025641BUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Chordin (chd), partial CSB-YP005377DIL
CSB-EP005377DIL
CSB-BP005377DIL
CSB-MP005377DIL
CSB-EP005377DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus horikoshii ADP-dependent glucokinase (glkA) CSB-YP001380FHX
CSB-EP001380FHX
CSB-BP001380FHX
CSB-MP001380FHX
CSB-EP001380FHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus horikoshii CTP synthase (pyrG), partial CSB-YP006176FHX
CSB-EP006176FHX
CSB-BP006176FHX
CSB-MP006176FHX
CSB-EP006176FHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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