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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Schizosaccharomyces pombe Adenylosuccinate lyase (ade8) CSB-YP001397SXV
CSB-EP001397SXV
CSB-BP001397SXV
CSB-MP001397SXV
CSB-EP001397SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Leucine carboxyl methyltransferase 2 (ppm2), partial CSB-YP012809SXV
CSB-EP012809SXV
CSB-BP012809SXV
CSB-MP012809SXV
CSB-EP012809SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delphinus delphis Rhodopsin (RHO), partial CSB-YP019681DJC1
CSB-EP019681DJC1
CSB-BP019681DJC1
CSB-MP019681DJC1
CSB-EP019681DJC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tursiops truncatus Rhodopsin (RHO), partial CSB-YP019681TRF1
CSB-EP019681TRF1
CSB-BP019681TRF1
CSB-MP019681TRF1
CSB-EP019681TRF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aquifex aeolicus Uroporphyrinogen decarboxylase (hemE) CSB-YP025678DNV
CSB-EP025678DNV
CSB-BP025678DNV
CSB-MP025678DNV
CSB-EP025678DNV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Cathepsin D (ctsD) CSB-YP006187DKK
CSB-EP006187DKK
CSB-BP006187DKK
CSB-MP006187DKK
CSB-EP006187DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Callithrix emiliae Beta-2-microglobulin (B2M) CSB-YP002486CYJ
CSB-EP002486CYJ
CSB-BP002486CYJ
CSB-MP002486CYJ
CSB-EP002486CYJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Callicebus personatus nigrifrons Beta-2-microglobulin (B2M) CSB-YP002486CYF
CSB-EP002486CYF
CSB-BP002486CYF
CSB-MP002486CYF
CSB-EP002486CYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saimiri boliviensis boliviensis Beta-2-microglobulin (B2M) CSB-YP002486SWC
CSB-EP002486SWC
CSB-BP002486SWC
CSB-MP002486SWC
CSB-EP002486SWC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorocebus aethiops Sterol O-acyltransferase 1 (SOAT1), partial CSB-YP022385DSU1
CSB-EP022385DSU1
CSB-BP022385DSU1
CSB-MP022385DSU1
CSB-EP022385DSU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays Defender against cell death 1 (DAD1), partial CSB-YP006487ZAX1
CSB-EP006487ZAX1
CSB-BP006487ZAX1
CSB-MP006487ZAX1
CSB-EP006487ZAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beta vulgaris Eukaryotic translation initiation factor 6 CSB-YP007582BSQ
CSB-EP007582BSQ
CSB-BP007582BSQ
CSB-MP007582BSQ
CSB-EP007582BSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coffea arabica Spermidine synthase CSB-YP022668DVA
CSB-EP022668DVA
CSB-BP022668DVA
CSB-MP022668DVA
CSB-EP022668DVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia trachomatis 6-phosphogluconolactonase (pgl) CSB-YP017861DSB
CSB-EP017861DSB
CSB-BP017861DSB
CSB-MP017861DSB
CSB-EP017861DSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Galactose-1-phosphate uridylyltransferase (galT) CSB-YP009226LMH
CSB-EP009226LMH
CSB-BP009226LMH
CSB-MP009226LMH
CSB-EP009226LMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae Tyrosine aminotransferase (tyrB) CSB-YP023175KBG
CSB-EP023175KBG
CSB-BP023175KBG
CSB-MP023175KBG
CSB-EP023175KBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria perflava Ornithine carbamoyltransferase (argF) CSB-YP017270NGJ
CSB-EP017270NGJ
CSB-BP017270NGJ
CSB-MP017270NGJ
CSB-EP017270NGJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria subflava Ornithine carbamoyltransferase (argF) CSB-YP017270NGM
CSB-EP017270NGM
CSB-BP017270NGM
CSB-MP017270NGM
CSB-EP017270NGM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida albicans Eukaryotic translation initiation factor 5A (ANB1) CSB-YP007573CZD
CSB-EP007573CZD
CSB-BP007573CZD
CSB-MP007573CZD
CSB-EP007573CZD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Borrelia burgdorferi CTP synthase (pyrG), partial CSB-YP006176BUD
CSB-EP006176BUD
CSB-BP006176BUD
CSB-MP006176BUD
CSB-EP006176BUD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務,您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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