Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Prochlorococcus marinus Uroporphyrinogen decarboxylase (hemE) CSB-YP025678PZF
CSB-EP025678PZF
CSB-BP025678PZF
CSB-MP025678PZF
CSB-EP025678PZF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Strongylocentrotus purpuratus Dual serine/threonine and tyrosine protein kinase (DSTYK), partial CSB-YP007213FOT
CSB-EP007213FOT
CSB-BP007213FOT
CSB-MP007213FOT
CSB-EP007213FOT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Transmembrane protein 213 (TMEM213), partial CSB-YP023807HU1
CSB-EP023807HU1
CSB-BP023807HU1
CSB-MP023807HU1
CSB-EP023807HU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Zinc finger protein 346 (znf346) CSB-YP026691DIL
CSB-EP026691DIL
CSB-BP026691DIL
CSB-MP026691DIL
CSB-EP026691DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Aminomethyltransferase (gcvT) CSB-YP001684BPP
CSB-EP001684BPP
CSB-BP001684BPP
CSB-MP001684BPP
CSB-EP001684BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei Uroporphyrinogen decarboxylase (hemE) CSB-YP025678BPP
CSB-EP025678BPP
CSB-BP025678BPP
CSB-MP025678BPP
CSB-EP025678BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei CTP synthase (pyrG), partial CSB-YP006176BPP
CSB-EP006176BPP
CSB-BP006176BPP
CSB-MP006176BPP
CSB-EP006176BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanocorpusculum labreanum Argininosuccinate lyase (argH) CSB-YP002213MNT
CSB-EP002213MNT
CSB-BP002213MNT
CSB-MP002213MNT
CSB-EP002213MNT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Zinc finger protein 16 (ZNF16), partial CSB-YP026548EQV
CSB-EP026548EQV
CSB-BP026548EQV
CSB-MP026548EQV
CSB-EP026548EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Mitogen-activated protein kinase 13 (MPK13), partial CSB-YP013452OFF
CSB-EP013452OFF
CSB-BP013452OFF
CSB-MP013452OFF
CSB-EP013452OFF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. indica Defender against cell death 1 (DAD1), partial CSB-YP006487OFF1
CSB-EP006487OFF1
CSB-BP006487OFF1
CSB-MP006487OFF1
CSB-EP006487OFF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus sanguinis Uracil-DNA glycosylase (ung) CSB-YP025641SUR
CSB-EP025641SUR
CSB-BP025641SUR
CSB-MP025641SUR
CSB-EP025641SUR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri CTP synthase (pyrG), partial CSB-YP006176MNU
CSB-EP006176MNU
CSB-BP006176MNU
CSB-MP006176MNU
CSB-EP006176MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Phosphoglycolate phosphatase (Memar_1919) CSB-YP017873MNU
CSB-EP017873MNU
CSB-BP017873MNU
CSB-MP017873MNU
CSB-EP017873MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanoculleus marisnigri Glyceraldehyde-3-phosphate dehydrogenase (gap) CSB-YP009232MNU
CSB-EP009232MNU
CSB-BP009232MNU
CSB-MP009232MNU
CSB-EP009232MNU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Cytidine deaminase (cdd) CSB-YP004976STL
CSB-EP004976STL
CSB-BP004976STL
CSB-MP004976STL
CSB-EP004976STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Hydroxyacylglutathione hydrolase (gloB) CSB-YP010121STL
CSB-EP010121STL
CSB-BP010121STL
CSB-MP010121STL
CSB-EP010121STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica CTP synthase (pyrG), partial CSB-YP006176STL
CSB-EP006176STL
CSB-BP006176STL
CSB-MP006176STL
CSB-EP006176STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Argininosuccinate lyase (argH) CSB-YP002213DUT
CSB-EP002213DUT
CSB-BP002213DUT
CSB-MP002213DUT
CSB-EP002213DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus Phosphoglycolate phosphatase (Smar_1152) CSB-YP017873SUP
CSB-EP017873SUP
CSB-BP017873SUP
CSB-MP017873SUP
CSB-EP017873SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>