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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Ochrobactrum anthropi 3-hydroxyanthranilate 3,4-dioxygenase (Oant_4402) CSB-YP010112ODH
CSB-EP010112ODH
CSB-BP010112ODH
CSB-MP010112ODH
CSB-EP010112ODH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Aminomethyltransferase (gcvT) CSB-YP001684YAN
CSB-EP001684YAN
CSB-BP001684YAN
CSB-MP001684YAN
CSB-EP001684YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pseudotuberculosis serotype O:1b Uracil-DNA glycosylase (ung) CSB-YP025641YAN
CSB-EP025641YAN
CSB-BP025641YAN
CSB-MP025641YAN
CSB-EP025641YAN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nitratiruptor sp. Uroporphyrinogen decarboxylase (hemE) CSB-YP025678NFA
CSB-EP025678NFA
CSB-BP025678NFA
CSB-MP025678NFA
CSB-EP025678NFA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae GTP cyclohydrolase 1 (folE) CSB-YP009317KAX
CSB-EP009317KAX
CSB-BP009317KAX
CSB-MP009317KAX
CSB-EP009317KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Klebsiella pneumoniae subsp. pneumoniae CTP synthase (pyrG), partial CSB-YP006176KAX
CSB-EP006176KAX
CSB-BP006176KAX
CSB-MP006176KAX
CSB-EP006176KAX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Alkaliphilus metalliredigens Adenine phosphoribosyltransferase (apt) CSB-YP001954AUP
CSB-EP001954AUP
CSB-BP001954AUP
CSB-MP001954AUP
CSB-EP001954AUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas aeruginosa Uroporphyrinogen decarboxylase (hemE) CSB-YP025678PZL
CSB-EP025678PZL
CSB-BP025678PZL
CSB-MP025678PZL
CSB-EP025678PZL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Marinomonas sp. Glutaminase (glsA) CSB-YP009528MNJ
CSB-EP009528MNJ
CSB-BP009528MNJ
CSB-MP009528MNJ
CSB-EP009528MNJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans Tryptophan 2,3-dioxygenase (kynA) CSB-YP023351KAW
CSB-EP023351KAW
CSB-BP023351KAW
CSB-MP023351KAW
CSB-EP023351KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kineococcus radiotolerans Adenine phosphoribosyltransferase (apt) CSB-YP001954KAW
CSB-EP001954KAW
CSB-BP001954KAW
CSB-MP001954KAW
CSB-EP001954KAW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Babesia bovis Eukaryotic translation initiation factor 6 (EIF6) CSB-YP007582BPY
CSB-EP007582BPY
CSB-BP007582BPY
CSB-MP007582BPY
CSB-EP007582BPY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sclerotinia sclerotiorum Adenylate kinase 1 (adk1) CSB-YP001508STG
CSB-EP001508STG
CSB-BP001508STG
CSB-MP001508STG
CSB-EP001508STG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Sulfurovum sp. Argininosuccinate lyase (argH) CSB-YP002213SUZ
CSB-EP002213SUZ
CSB-BP002213SUZ
CSB-MP002213SUZ
CSB-EP002213SUZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ornithine carbamoyltransferase (argF) CSB-YP017270FLG
CSB-EP017270FLG
CSB-BP017270FLG
CSB-MP017270FLG
CSB-EP017270FLG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Transmembrane protein 163 (TMEM163), partial CSB-YP023737BO1
CSB-EP023737BO1
CSB-BP023737BO1
CSB-MP023737BO1
CSB-EP023737BO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana Adenylate kinase 1 (adk1) CSB-YP001508BVD
CSB-EP001508BVD
CSB-BP001508BVD
CSB-MP001508BVD
CSB-EP001508BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana Phosphatidylethanolamine N-methyltransferase (CHO2), partial CSB-YP017780BVD
CSB-EP017780BVD
CSB-BP017780BVD
CSB-MP017780BVD
CSB-EP017780BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Botryotinia fuckeliana Mitochondrial intermediate peptidase (oct1), partial CSB-YP013835BVD
CSB-EP013835BVD
CSB-BP013835BVD
CSB-MP013835BVD
CSB-EP013835BVD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Janthinobacterium sp. Argininosuccinate lyase (argH) CSB-YP002213JAQ
CSB-EP002213JAQ
CSB-BP002213JAQ
CSB-MP002213JAQ
CSB-EP002213JAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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