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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Azorhizobium caulinodans Glutaminase (glsA) CSB-YP009528DPR
CSB-EP009528DPR
CSB-BP009528DPR
CSB-MP009528DPR
CSB-EP009528DPR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dinoroseobacter shibae Argininosuccinate lyase (argH) CSB-YP002213DIB
CSB-EP002213DIB
CSB-BP002213DIB
CSB-MP002213DIB
CSB-EP002213DIB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter concisus Uroporphyrinogen decarboxylase (hemE) CSB-YP025678CWA
CSB-EP025678CWA
CSB-BP025678CWA
CSB-MP025678CWA
CSB-EP025678CWA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Spermidine synthase (speE) CSB-YP022668EJD
CSB-EP022668EJD
CSB-BP022668EJD
CSB-MP022668EJD
CSB-EP022668EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Choline dehydrogenase (betA) CSB-YP005335EJD
CSB-EP005335EJD
CSB-BP005335EJD
CSB-MP005335EJD
CSB-EP005335EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 CTP synthase (pyrG) CSB-YP006176EJD
CSB-EP006176EJD
CSB-BP006176EJD
CSB-MP006176EJD
CSB-EP006176EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O139:H28 Uroporphyrinogen decarboxylase (hemE) CSB-YP025678EJD
CSB-EP025678EJD
CSB-BP025678EJD
CSB-MP025678EJD
CSB-EP025678EJD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Spermidine synthase (speE) CSB-YP022668EJF
CSB-EP022668EJF
CSB-BP022668EJF
CSB-MP022668EJF
CSB-EP022668EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Adenosine deaminase (add) CSB-YP001268EJF
CSB-EP001268EJF
CSB-BP001268EJF
CSB-MP001268EJF
CSB-EP001268EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 GTP cyclohydrolase 1 (folE) CSB-YP009317EJF
CSB-EP009317EJF
CSB-BP009317EJF
CSB-MP009317EJF
CSB-EP009317EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 CTP synthase (pyrG) CSB-YP006176EJF
CSB-EP006176EJF
CSB-BP006176EJF
CSB-MP006176EJF
CSB-EP006176EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O9:H4 Aminomethyltransferase (gcvT) CSB-YP001684EJF
CSB-EP001684EJF
CSB-BP001684EJF
CSB-MP001684EJF
CSB-EP001684EJF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri Uracil-DNA glycosylase (ung) CSB-YP025641CWS
CSB-EP025641CWS
CSB-BP025641CWS
CSB-MP025641CWS
CSB-EP025641CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Citrobacter koseri CTP synthase (pyrG), partial CSB-YP006176CWS
CSB-EP006176CWS
CSB-BP006176CWS
CSB-MP006176CWS
CSB-EP006176CWS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus gordonii Uracil-DNA glycosylase (ung) CSB-YP025641FMN
CSB-EP025641FMN
CSB-BP025641FMN
CSB-MP025641FMN
CSB-EP025641FMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arcobacter butzleri Adenine phosphoribosyltransferase (apt) CSB-YP001954AUY
CSB-EP001954AUY
CSB-BP001954AUY
CSB-MP001954AUY
CSB-EP001954AUY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rickettsia canadensis Uroporphyrinogen decarboxylase (hemE) CSB-YP025678RMR
CSB-EP025678RMR
CSB-BP025678RMR
CSB-MP025678RMR
CSB-EP025678RMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus pumilus Uroporphyrinogen decarboxylase (hemE) CSB-YP025678BOL
CSB-EP025678BOL
CSB-BP025678BOL
CSB-MP025678BOL
CSB-EP025678BOL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Campylobacter jejuni subsp. jejuni serotype O:6 Uracil-DNA glycosylase (ung) CSB-YP025641CWG
CSB-EP025641CWG
CSB-BP025641CWG
CSB-MP025641CWG
CSB-EP025641CWG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sediminis Cytidine deaminase (cdd) CSB-YP004976STR
CSB-EP004976STR
CSB-BP004976STR
CSB-MP004976STR
CSB-EP004976STR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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