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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Thermoanaerobacterium thermosaccharolyticum Electron transfer flavoprotein subunit alpha (etfA) CSB-YP007844TML
CSB-EP007844TML
CSB-BP007844TML
CSB-MP007844TML
CSB-EP007844TML-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma capricolum subsp. capricolum Peptide chain release factor 1 (prfA) CSB-YP015197MLJ
CSB-EP015197MLJ
CSB-BP015197MLJ
CSB-MP015197MLJ
CSB-EP015197MLJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trehalase (Rv2402), partial CSB-YP024402MVZ
CSB-EP024402MVZ
CSB-BP024402MVZ
CSB-MP024402MVZ
CSB-EP024402MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Translation initiation factor IF-3 (infC) CSB-YP015166SSQ
CSB-EP015166SSQ
CSB-BP015166SSQ
CSB-MP015166SSQ
CSB-EP015166SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Glycine cleavage system H protein (gcvH) CSB-YP009335SSQ
CSB-EP009335SSQ
CSB-BP009335SSQ
CSB-MP009335SSQ
CSB-EP009335SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Methylthioribose-1-phosphate isomerase (mtnA) CSB-YP014799SSQ
CSB-EP014799SSQ
CSB-BP014799SSQ
CSB-MP014799SSQ
CSB-EP014799SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium radiobacter Elongation factor Tu (tufA) CSB-YP025342RKV
CSB-EP025342RKV
CSB-BP025342RKV
CSB-MP025342RKV
CSB-EP025342RKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Pyruvate dehydrogenase E1 component subunit alpha (pdhA) CSB-YP017716MLW
CSB-EP017716MLW
CSB-BP017716MLW
CSB-MP017716MLW
CSB-EP017716MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Thioredoxin reductase (trxB) CSB-YP025388MLW
CSB-EP025388MLW
CSB-BP025388MLW
CSB-MP025388MLW
CSB-EP025388MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli NADP-dependent malic enzyme (maeB) CSB-YP013634ENV
CSB-EP013634ENV
CSB-BP013634ENV
CSB-MP013634ENV
CSB-EP013634ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Delta-aminolevulinic acid dehydratase (hemB) CSB-YP001558EYU
CSB-EP001558EYU
CSB-BP001558EYU
CSB-MP001558EYU
CSB-EP001558EYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Glutamine synthetase (glnA) CSB-YP009553SSQ
CSB-EP009553SSQ
CSB-BP009553SSQ
CSB-MP009553SSQ
CSB-EP009553SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Delta-aminolevulinic acid dehydratase (hemB) CSB-YP001558SSQ
CSB-EP001558SSQ
CSB-BP001558SSQ
CSB-MP001558SSQ
CSB-EP001558SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Elongation factor Ts (tsf) CSB-YP025124MLW
CSB-EP025124MLW
CSB-BP025124MLW
CSB-MP025124MLW
CSB-EP025124MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Thymidylate synthase (thyA) CSB-YP025393MLW
CSB-EP025393MLW
CSB-BP025393MLW
CSB-MP025393MLW
CSB-EP025393MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Agaricus bisporus Delta-1-pyrroline-5-carboxylate dehydrogenase (pruA), partial CSB-YP001576AYJ
CSB-EP001576AYJ
CSB-BP001576AYJ
CSB-MP001576AYJ
CSB-EP001576AYJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neurospora crassa Actin (act-1) CSB-YP001205NHA
CSB-EP001205NHA
CSB-BP001205NHA
CSB-MP001205NHA
CSB-EP001205NHA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Urocanate hydratase (hutU), partial CSB-YP025677SKY
CSB-EP025677SKY
CSB-BP025677SKY
CSB-MP025677SKY
CSB-EP025677SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Choristoneura pinus Cytochrome c oxidase subunit 1 (COI), partial CSB-YP015072CJW
CSB-EP015072CJW
CSB-BP015072CJW
CSB-MP015072CJW
CSB-EP015072CJW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bos gaurus frontalis Hemoglobin subunit beta (HBB) CSB-YP010150BIR
CSB-EP010150BIR
CSB-BP010150BIR
CSB-MP010150BIR
CSB-EP010150BIR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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