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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Streptococcus pyogenes GTP cyclohydrolase 1 (folE) CSB-YP009317SMR
CSB-EP009317SMR
CSB-BP009317SMR
CSB-MP009317SMR
CSB-EP009317SMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes Glyceraldehyde-3-phosphate dehydrogenase (gap) CSB-YP009232SMR
CSB-EP009232SMR
CSB-BP009232SMR
CSB-MP009232SMR
CSB-EP009232SMR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia trachomatis Glyceraldehyde-3-phosphate dehydrogenase (gap) CSB-YP009232DSB
CSB-EP009232DSB
CSB-BP009232DSB
CSB-MP009232DSB
CSB-EP009232DSB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Caulobacter crescentus Uroporphyrinogen decarboxylase (hemE) CSB-YP025678DQO
CSB-EP025678DQO
CSB-BP025678DQO
CSB-MP025678DQO
CSB-EP025678DQO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Galactose-1-phosphate uridylyltransferase (GAL7) CSB-YP009226CTK
CSB-EP009226CTK
CSB-BP009226CTK
CSB-MP009226CTK
CSB-EP009226CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coccidioides posadasii 4-hydroxyphenylpyruvate dioxygenase (TCRP) CSB-YP010698DUW
CSB-EP010698DUW
CSB-BP010698DUW
CSB-MP010698DUW
CSB-EP010698DUW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Regulator of telomere elongation helicase 1 (rtel1), partial CSB-YP020563DIL
CSB-EP020563DIL
CSB-BP020563DIL
CSB-MP020563DIL
CSB-EP020563DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Adenylate kinase 1 (ADK1) CSB-YP001508CTK
CSB-EP001508CTK
CSB-BP001508CTK
CSB-MP001508CTK
CSB-EP001508CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Crotalus atrox Glutaminyl-peptide cyclotransferase (QPCT) CSB-YP019135DYC
CSB-EP019135DYC
CSB-BP019135DYC
CSB-MP019135DYC
CSB-EP019135DYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus Transketolase (tktA), partial CSB-YP023580RLC
CSB-EP023580RLC
CSB-BP023580RLC
CSB-MP023580RLC
CSB-EP023580RLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Adenine phosphoribosyltransferase (apt) CSB-YP001954SMW
CSB-EP001954SMW
CSB-BP001954SMW
CSB-MP001954SMW
CSB-EP001954SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 GTP cyclohydrolase 1 (folE) CSB-YP009317SMV
CSB-EP009317SMV
CSB-BP009317SMV
CSB-MP009317SMV
CSB-EP009317SMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis Pyrroline-5-carboxylate reductase 1 (proH) CSB-YP019115BRJ
CSB-EP019115BRJ
CSB-BP019115BRJ
CSB-MP019115BRJ
CSB-EP019115BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D 3-hydroxyanthranilate 3,4-dioxygenase (BNA1) CSB-YP010112CTK
CSB-EP010112CTK
CSB-BP010112CTK
CSB-MP010112CTK
CSB-EP010112CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Glyceraldehyde-3-phosphate dehydrogenase (GPD) CSB-YP009232CTL
CSB-EP009232CTL
CSB-BP009232CTL
CSB-MP009232CTL
CSB-EP009232CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Protein phosphatase methylesterase 1 (PPE1) CSB-YP018501CTK
CSB-EP018501CTK
CSB-BP018501CTK
CSB-MP018501CTK
CSB-EP018501CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pyogenes serotype M3 Glyceraldehyde-3-phosphate dehydrogenase (gap) CSB-YP009232SMW
CSB-EP009232SMW
CSB-BP009232SMW
CSB-MP009232SMW
CSB-EP009232SMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Glyceraldehyde-3-phosphate dehydrogenase (GPD) CSB-YP009232CTK
CSB-EP009232CTK
CSB-BP009232CTK
CSB-MP009232CTK
CSB-EP009232CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Leucine carboxyl methyltransferase 1 (PPM1) CSB-YP012808CTK
CSB-EP012808CTK
CSB-BP012808CTK
CSB-MP012808CTK
CSB-EP012808CTK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cryptococcus neoformans var. neoformans serotype D Protein phosphatase methylesterase 1 (PPE1) CSB-YP018501CTL
CSB-EP018501CTL
CSB-BP018501CTL
CSB-MP018501CTL
CSB-EP018501CTL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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