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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Histone H3.2 CSB-YP010419TBW
CSB-EP010419TBW
CSB-BP010419TBW
CSB-MP010419TBW
CSB-EP010419TBW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pangasius pangasius Somatotropin (gh) CSB-YP009407PAY
CSB-EP009407PAY
CSB-BP009407PAY
CSB-MP009407PAY
CSB-EP009407PAY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cichlasoma labiatum Cytochrome b (mt-cyb), partial CSB-YP015075CKN1
CSB-EP015075CKN1
CSB-BP015075CKN1
CSB-MP015075CKN1
CSB-EP015075CKN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Branchiostoma floridae NADH-ubiquinone oxidoreductase chain 6 (ND6), partial CSB-YP015082BVY1
CSB-EP015082BVY1
CSB-BP015082BVY1
CSB-MP015082BVY1
CSB-EP015082BVY1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Branchiostoma lanceolatum NADH-ubiquinone oxidoreductase chain 4L (ND4L), partial CSB-YP015080BVZ1
CSB-EP015080BVZ1
CSB-BP015080BVZ1
CSB-MP015080BVZ1
CSB-EP015080BVZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus clarkii NADH-ubiquinone oxidoreductase chain 4L (MT-ND4L), partial CSB-YP015080OBE1
CSB-EP015080OBE1
CSB-BP015080OBE1
CSB-MP015080OBE1
CSB-EP015080OBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Hemoglobin subunit alpha (HBA1) CSB-YP010147EQV
CSB-EP010147EQV
CSB-BP010147EQV
CSB-MP010147EQV
CSB-EP010147EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leucoraja ocellata Gap junction delta-2 protein, partial CSB-YP009461RAP1
CSB-EP009461RAP1
CSB-BP009461RAP1
CSB-MP009461RAP1
CSB-EP009461RAP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus Kinesin-like protein KIF2C (KIF2C), partial CSB-YP012334DXU
CSB-EP012334DXU
CSB-BP012334DXU
CSB-MP012334DXU
CSB-EP012334DXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlamydia muridarum Ribosome-recycling factor (frr) CSB-YP014941CIU
CSB-EP014941CIU
CSB-BP014941CIU
CSB-MP014941CIU
CSB-EP014941CIU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ferrochelatase (hemH) CSB-YP008579EYU
CSB-EP008579EYU
CSB-BP008579EYU
CSB-MP008579EYU
CSB-EP008579EYU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor Tu (tuf) CSB-YP025342PLD
CSB-EP025342PLD
CSB-BP025342PLD
CSB-MP025342PLD
CSB-EP025342PLD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter capsulatus ATP synthase subunit delta (atpH) CSB-YP002355RLC
CSB-EP002355RLC
CSB-BP002355RLC
CSB-MP002355RLC
CSB-EP002355RLC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodobacter sphaeroides Alcohol dehydrogenase class-3 (adhI) CSB-YP001357RLF
CSB-EP001357RLF
CSB-BP001357RLF
CSB-MP001357RLF
CSB-EP001357RLF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Transaldolase (tal) CSB-YP023112SSQ
CSB-EP023112SSQ
CSB-BP023112SSQ
CSB-MP023112SSQ
CSB-EP023112SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. DNA topoisomerase 1 (topA), partial CSB-YP024056SSQ
CSB-EP024056SSQ
CSB-BP024056SSQ
CSB-MP024056SSQ
CSB-EP024056SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechocystis sp. Glycerol-3-phosphate dehydrogenase (glpD), partial CSB-YP009711SSQ
CSB-EP009711SSQ
CSB-BP009711SSQ
CSB-MP009711SSQ
CSB-EP009711SSQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Thymidylate kinase (tmk) CSB-YP007226MLW
CSB-EP007226MLW
CSB-BP007226MLW
CSB-MP007226MLW
CSB-EP007226MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae GTPase Era (era) CSB-YP007759MLW
CSB-EP007759MLW
CSB-BP007759MLW
CSB-MP007759MLW
CSB-EP007759MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycoplasma pneumoniae Inorganic pyrophosphatase (ppa) CSB-YP018410MLW
CSB-EP018410MLW
CSB-BP018410MLW
CSB-MP018410MLW
CSB-EP018410MLW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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