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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Saccharomyces cerevisiae Prefoldin subunit 2 (GIM4) CSB-YP017813SVG
CSB-EP017813SVG
CSB-BP017813SVG
CSB-MP017813SVG
CSB-EP017813SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Argininosuccinate lyase (arg7) CSB-YP002213SXV
CSB-EP002213SXV
CSB-BP002213SXV
CSB-MP002213SXV
CSB-EP002213SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces douglasii Argininosuccinate lyase (ARG4) CSB-YP002213SVS
CSB-EP002213SVS
CSB-BP002213SVS
CSB-MP002213SVS
CSB-EP002213SVS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Insulin-degrading enzyme (Ide), partial CSB-YP010988DLU
CSB-EP010988DLU
CSB-BP010988DLU
CSB-MP010988DLU
CSB-EP010988DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Loligo forbesi Rhodopsin (RHO), partial CSB-YP019681LHZ1
CSB-EP019681LHZ1
CSB-BP019681LHZ1
CSB-MP019681LHZ1
CSB-EP019681LHZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cebus albifrons Involucrin (IVL) CSB-YP011922CFJ
CSB-EP011922CFJ
CSB-BP011922CFJ
CSB-MP011922CFJ
CSB-EP011922CFJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Serratia odorifera Glyceraldehyde-3-phosphate dehydrogenase (gap) CSB-YP009232SFL
CSB-EP009232SFL
CSB-BP009232SFL
CSB-MP009232SFL
CSB-EP009232SFL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Raja clavata Brain-derived neurotrophic factor (bdnf) CSB-YP002655RAO
CSB-EP002655RAO
CSB-BP002655RAO
CSB-MP002655RAO
CSB-EP002655RAO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mycobacterium smegmatis Citrate synthase (gltA) CSB-YP006031MVW
CSB-EP006031MVW
CSB-BP006031MVW
CSB-MP006031MVW
CSB-EP006031MVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Mitogen-activated protein kinase 1 (mapk1) CSB-YP013448XBE
CSB-EP013448XBE
CSB-BP013448XBE
CSB-MP013448XBE
CSB-EP013448XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Flavobacterium meningosepticum Prolyl endopeptidase (f1pep1), partial CSB-YP018664FEW
CSB-EP018664FEW
CSB-BP018664FEW
CSB-MP018664FEW
CSB-EP018664FEW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Interferon-induced protein 44 (IFI44) CSB-YP011014EQV
CSB-EP011014EQV
CSB-BP011014EQV
CSB-MP011014EQV
CSB-EP011014EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 2 Uracil-DNA glycosylase (UL2) CSB-YP025641HJX
CSB-EP025641HJX
CSB-BP025641HJX
CSB-MP025641HJX
CSB-EP025641HJX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cochliobolus lunatus Glyceraldehyde-3-phosphate dehydrogenase (GPD) CSB-YP009232CMV
CSB-EP009232CMV
CSB-BP009232CMV
CSB-MP009232CMV
CSB-EP009232CMV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Amia calva Insulin (ins) CSB-YP011742AIW
CSB-EP011742AIW
CSB-BP011742AIW
CSB-MP011742AIW
CSB-EP011742AIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Rhodopsin (rho), partial CSB-YP019681XBE1
CSB-EP019681XBE1
CSB-BP019681XBE1
CSB-MP019681XBE1
CSB-EP019681XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Erythropoietin (Epo) CSB-YP007743RA
CSB-EP007743RA
CSB-BP007743RA
CSB-MP007743RA
CSB-EP007743RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Trachydosaurus rugosus Transthyretin (TTR) CSB-YP025270THD
CSB-EP025270THD
CSB-BP025270THD
CSB-MP025270THD
CSB-EP025270THD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Exoribonuclease 2 (rnb) CSB-YP007787ENV
CSB-EP007787ENV
CSB-BP007787ENV
CSB-MP007787ENV
CSB-EP007787ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Bone morphogenetic protein 4 (bmp4) CSB-YP002740XBE
CSB-EP002740XBE
CSB-BP002740XBE
CSB-MP002740XBE
CSB-EP002740XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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