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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Pasteurella multocida Ornithine carbamoyltransferase (argF) CSB-YP017270ESG
CSB-EP017270ESG
CSB-BP017270ESG
CSB-MP017270ESG
CSB-EP017270ESG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Helicobacter pylori Adenylosuccinate lyase (purB) CSB-YP001397HUV
CSB-EP001397HUV
CSB-BP001397HUV
CSB-MP001397HUV
CSB-EP001397HUV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rana temporaria Rhodopsin (RHO), partial CSB-YP019681RBJ1
CSB-EP019681RBJ1
CSB-BP019681RBJ1
CSB-MP019681RBJ1
CSB-EP019681RBJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Adenylosuccinate lyase (purB) CSB-YP001397BWZ
CSB-EP001397BWZ
CSB-BP001397BWZ
CSB-MP001397BWZ
CSB-EP001397BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Glutathione synthetase (gshB) CSB-YP009969BWZ
CSB-EP009969BWZ
CSB-BP009969BWZ
CSB-MP009969BWZ
CSB-EP009969BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ralstonia solanacearum Glutathione synthetase (gshB) CSB-YP009969RAR
CSB-EP009969RAR
CSB-BP009969RAR
CSB-MP009969RAR
CSB-EP009969RAR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhi Glutathione synthetase (gshB) CSB-YP009969SWW
CSB-EP009969SWW
CSB-BP009969SWW
CSB-MP009969SWW
CSB-EP009969SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus Lymphocyte antigen 96 (LY96) CSB-YP013254DXU
CSB-EP013254DXU
CSB-BP013254DXU
CSB-MP013254DXU
CSB-EP013254DXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chlorobium tepidum CTP synthase (pyrG), partial CSB-YP006176DST
CSB-EP006176DST
CSB-BP006176DST
CSB-MP006176DST
CSB-EP006176DST-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri Exoribonuclease 2 (rnb), partial CSB-YP007787SZB
CSB-EP007787SZB
CSB-BP007787SZB
CSB-MP007787SZB
CSB-EP007787SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Glutathione synthetase (gshB) CSB-YP009969BMZ
CSB-EP009969BMZ
CSB-BP009969BMZ
CSB-MP009969BMZ
CSB-EP009969BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae CTP synthase (pyrG), partial CSB-YP006176BMZ
CSB-EP006176BMZ
CSB-BP006176BMZ
CSB-MP006176BMZ
CSB-EP006176BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia multivorans Argininosuccinate lyase (argH) CSB-YP002213BPS
CSB-EP002213BPS
CSB-BP002213BPS
CSB-MP002213BPS
CSB-EP002213BPS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium tetani Argininosuccinate lyase (argH) CSB-YP002213CMN
CSB-EP002213CMN
CSB-BP002213CMN
CSB-MP002213CMN
CSB-EP002213CMN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus plantarum Argininosuccinate lyase (argH) CSB-YP002213LMS
CSB-EP002213LMS
CSB-BP002213LMS
CSB-MP002213LMS
CSB-EP002213LMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri Argininosuccinate lyase (argH) CSB-YP002213SZB
CSB-EP002213SZB
CSB-BP002213SZB
CSB-MP002213SZB
CSB-EP002213SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae GTP cyclohydrolase 1 (folE) CSB-YP009317SMP
CSB-EP009317SMP
CSB-BP009317SMP
CSB-MP009317SMP
CSB-EP009317SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Glycerate kinase (glxK) CSB-YP009563NGF
CSB-EP009563NGF
CSB-BP009563NGF
CSB-MP009563NGF
CSB-EP009563NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Thioredoxin (trxA) CSB-YP025365BWZ
CSB-EP025365BWZ
CSB-BP025365BWZ
CSB-MP025365BWZ
CSB-EP025365BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pasteurella multocida Argininosuccinate lyase (argH) CSB-YP002213ESG
CSB-EP002213ESG
CSB-BP002213ESG
CSB-MP002213ESG
CSB-EP002213ESG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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