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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Streptococcus pyogenes serotype M1 Histidine ammonia-lyase (hutH), partial CSB-YP010123SMT
CSB-EP010123SMT
CSB-BP010123SMT
CSB-MP010123SMT
CSB-EP010123SMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bufo marinus Rhodopsin (RHO), partial CSB-YP019681BXI1
CSB-EP019681BXI1
CSB-BP019681BXI1
CSB-MP019681BXI1
CSB-EP019681BXI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Myxococcus xanthus Protoporphyrinogen oxidase (hemY) CSB-YP018502MXA
CSB-EP018502MXA
CSB-BP018502MXA
CSB-MP018502MXA
CSB-EP018502MXA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Transketolase (tkt), partial CSB-YP023580BWZ
CSB-EP023580BWZ
CSB-BP023580BWZ
CSB-MP023580BWZ
CSB-EP023580BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Hydroxyacylglutathione hydrolase (gloB) CSB-YP010121BWZ
CSB-EP010121BWZ
CSB-BP010121BWZ
CSB-MP010121BWZ
CSB-EP010121BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Glyceraldehyde-3-phosphate dehydrogenase (gapA) CSB-YP009232BWZ
CSB-EP009232BWZ
CSB-BP009232BWZ
CSB-MP009232BWZ
CSB-EP009232BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Acyrthosiphon pisum Ornithine carbamoyltransferase (argI) CSB-YP017270BWZ
CSB-EP017270BWZ
CSB-BP017270BWZ
CSB-MP017270BWZ
CSB-EP017270BWZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Glutathione synthetase (gshB) CSB-YP009969EOD
CSB-EP009969EOD
CSB-BP009969EOD
CSB-MP009969EOD
CSB-EP009969EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Glutathione synthetase (gshB) CSB-YP009969SXB
CSB-EP009969SXB
CSB-BP009969SXB
CSB-MP009969SXB
CSB-EP009969SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yersinia pestis Glutathione synthetase (gshB) CSB-YP009969YAS
CSB-EP009969YAS
CSB-BP009969YAS
CSB-MP009969YAS
CSB-EP009969YAS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Coturnix coturnix japonica Nuclear RNA export factor 1 (NXF1), partial CSB-YP016218DXJ
CSB-EP016218DXJ
CSB-BP016218DXJ
CSB-MP016218DXJ
CSB-EP016218DXJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Methanopyrus kandleri Glyceraldehyde-3-phosphate dehydrogenase (gap) CSB-YP009232MSI
CSB-EP009232MSI
CSB-BP009232MSI
CSB-MP009232MSI
CSB-EP009232MSI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salvelinus alpinus Steroidogenic acute regulatory protein (star) CSB-YP022798SBW
CSB-EP022798SBW
CSB-BP022798SBW
CSB-MP022798SBW
CSB-EP022798SBW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum CTP synthase (pyrG), partial CSB-YP006176BXE
CSB-EP006176BXE
CSB-BP006176BXE
CSB-MP006176BXE
CSB-EP006176BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Schizaphis graminum Ornithine carbamoyltransferase (argI) CSB-YP017270BXE
CSB-EP017270BXE
CSB-BP017270BXE
CSB-MP017270BXE
CSB-EP017270BXE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Uracil-DNA glycosylase (ung) CSB-YP025641BMZ
CSB-EP025641BMZ
CSB-BP025641BMZ
CSB-MP025641BMZ
CSB-EP025641BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Thioredoxin (trxA) CSB-YP025365BMZ
CSB-EP025365BMZ
CSB-BP025365BMZ
CSB-MP025365BMZ
CSB-EP025365BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum Argininosuccinate lyase (argH) CSB-YP002213BVW
CSB-EP002213BVW
CSB-BP002213BVW
CSB-MP002213BVW
CSB-EP002213BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Buchnera aphidicola subsp. Baizongia pistaciae Argininosuccinate lyase (argH) CSB-YP002213BMZ
CSB-EP002213BMZ
CSB-BP002213BMZ
CSB-MP002213BMZ
CSB-EP002213BMZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pseudomonas putida Argininosuccinate lyase (argH) CSB-YP002213FGB
CSB-EP002213FGB
CSB-BP002213FGB
CSB-MP002213FGB
CSB-EP002213FGB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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