Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Shigella flexneri CTP synthase (pyrG), partial CSB-YP006176SZB
CSB-EP006176SZB
CSB-BP006176SZB
CSB-MP006176SZB
CSB-EP006176SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Ribokinase (rbsK) CSB-YP019397ENV
CSB-BP019397ENV
CSB-MP019397ENV
CSB-EP019397ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Adenylosuccinate lyase (purB) CSB-YP001397ENV
CSB-EP001397ENV
CSB-BP001397ENV
CSB-MP001397ENV
CSB-EP001397ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Cytidine deaminase (cdd) CSB-YP004976ENV
CSB-EP004976ENV
CSB-BP004976ENV
CSB-MP004976ENV
CSB-EP004976ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Citrate synthase (gltA) CSB-YP006031ENV
CSB-EP006031ENV
CSB-BP006031ENV
CSB-MP006031ENV
CSB-EP006031ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Dihydrofolate reductase (folA) CSB-YP006847ENV
CSB-BP006847ENV
CSB-MP006847ENV
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri Dihydrofolate reductase (folA) CSB-YP006847SZB
CSB-EP006847SZB
CSB-BP006847SZB
CSB-MP006847SZB
CSB-EP006847SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Hydroxyacylglutathione hydrolase (gloB) CSB-YP010121ENV
CSB-EP010121ENV
CSB-BP010121ENV
CSB-MP010121ENV
CSB-EP010121ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O157:H7 Hydroxyacylglutathione hydrolase (gloB) CSB-YP010121EOD
CSB-EP010121EOD
CSB-BP010121EOD
CSB-MP010121EOD
CSB-EP010121EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli O6 GDP-mannose 4,6-dehydratase (gmd) CSB-YP009569EGX
CSB-EP009569EGX
CSB-BP009569EGX
CSB-MP009569EGX
CSB-EP009569EGX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella flexneri GDP-mannose 4,6-dehydratase (gmd) CSB-YP009569SZB
CSB-EP009569SZB
CSB-BP009569SZB
CSB-MP009569SZB
CSB-EP009569SZB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cricetulus griseus 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR), partial CSB-YP010565DXU
CSB-EP010565DXU
CSB-BP010565DXU
CSB-MP010565DXU
CSB-EP010565DXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Homarus americanus Glyceraldehyde-3-phosphate dehydrogenase CSB-YP009232HWO
CSB-EP009232HWO
CSB-BP009232HWO
CSB-MP009232HWO
CSB-EP009232HWO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacillus stearothermophilus Glyceraldehyde-3-phosphate dehydrogenase (gap) CSB-YP009232GFM
CSB-EP009232GFM
CSB-BP009232GFM
CSB-MP009232GFM
CSB-EP009232GFM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactobacillus casei Dihydrofolate reductase (folA) CSB-YP006847LMH
CSB-EP006847LMH
CSB-BP006847LMH
CSB-MP006847LMH
CSB-EP006847LMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Astacus fluviatilis Trypsin-1 CSB-YP018811APX
CSB-EP018811APX
CSB-BP018811APX
CSB-MP018811APX
CSB-EP018811APX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kirsten murine sarcoma virus GTPase KRas (K-RAS) CSB-YP012493KAG
CSB-EP012493KAG
CSB-BP012493KAG
CSB-MP012493KAG
CSB-EP012493KAG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acomys cahirinus Insulin (INS) CSB-YP011742ACC
CSB-EP011742ACC
CSB-BP011742ACC
CSB-MP011742ACC
CSB-EP011742ACC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chinchilla chinchilla Insulin (INS) CSB-YP011742CIK
CSB-EP011742CIK
CSB-BP011742CIK
CSB-MP011742CIK
CSB-EP011742CIK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Proechimys guairae Insulin (INS) CSB-YP011742PSE
CSB-EP011742PSE
CSB-BP011742PSE
CSB-MP011742PSE
CSB-EP011742PSE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>