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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant UDP-glucose 4-epimerase (galE) CSB-YP009197FMR
CSB-EP009197FMR
CSB-BP009197FMR
CSB-MP009197FMR
CSB-EP009197FMR-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus Inter-alpha-trypsin inhibitor heavy chain H2 (ITIH2), partial CSB-YP011895MRG
CSB-EP011895MRG
CSB-BP011895MRG
CSB-MP011895MRG
CSB-EP011895MRG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phodopus sungorus Thyrotroph embryonic factor (TEF) CSB-YP023373EUT
CSB-EP023373EUT
CSB-BP023373EUT
CSB-MP023373EUT
CSB-EP023373EUT-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Peptidyl-prolyl cis-trans isomerase FKBP1B (Fkbp1b) CSB-YP008692RA
CSB-EP008692RA
CSB-BP008692RA
CSB-MP008692RA
CSB-EP008692RA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Peroxisomal acyl-coenzyme A oxidase 2 (Acox2) CSB-YP001173RA
CSB-EP001173RA
CSB-BP001173RA
CSB-MP001173RA
CSB-EP001173RA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Calpain-1 catalytic subunit (Capn1) CSB-YP004490RA
CSB-EP004490RA
CSB-BP004490RA
CSB-MP004490RA
CSB-EP004490RA-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Zona pellucida sperm-binding protein 3 (Zp3), partial CSB-YP027121RA1
CSB-EP027121RA1
CSB-BP027121RA1
CSB-MP027121RA1
CSB-EP027121RA1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytochrome c oxidase subunit 1 (COI), partial CSB-YP015072CSG
CSB-EP015072CSG
CSB-BP015072CSG
CSB-MP015072CSG
CSB-EP015072CSG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Anas platyrhynchos Cytochrome c oxidase subunit 2 (MT-CO2), partial CSB-YP015073BZD1
CSB-EP015073BZD1
CSB-BP015073BZD1
CSB-MP015073BZD1
CSB-EP015073BZD1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytochrome c oxidase subunit 2 (COXII), partial CSB-YP015073TQY1
CSB-EP015073TQY1
CSB-BP015073TQY1
CSB-MP015073TQY1
CSB-EP015073TQY1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eulemur macaco Cytochrome c oxidase subunit 2 (MT-CO2), partial CSB-YP015073EJG1
CSB-EP015073EJG1
CSB-BP015073EJG1
CSB-MP015073EJG1
CSB-EP015073EJG1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tarsius bancanus Cytochrome c oxidase subunit 2 (MT-CO2), partial CSB-YP015073TLI1
CSB-EP015073TLI1
CSB-BP015073TLI1
CSB-MP015073TLI1
CSB-EP015073TLI1-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Adenylate cyclase type 9 (adcy9), partial CSB-YP001345XBE
CSB-EP001345XBE
CSB-BP001345XBE
CSB-MP001345XBE
CSB-EP001345XBE-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Nucleoside diphosphate kinase (ndk) CSB-YP015889SKY
CSB-EP015889SKY
CSB-BP015889SKY
CSB-MP015889SKY
CSB-EP015889SKY-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus ATP synthase subunit delta (atpH) CSB-YP002355SKY
CSB-EP002355SKY
CSB-BP002355SKY
CSB-MP002355SKY
CSB-EP002355SKY-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Isocitrate dehydrogenase [NADP] (icd) CSB-YP010990SKY
CSB-EP010990SKY
CSB-BP010990SKY
CSB-MP010990SKY
CSB-EP010990SKY-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mytilus edulis Cytochrome b (MT-CYB), partial CSB-YP015075MWU
CSB-EP015075MWU
CSB-BP015075MWU
CSB-MP015075MWU
CSB-EP015075MWU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mytilus edulis NADH-ubiquinone oxidoreductase chain 5 (ND5), partial CSB-YP015081MWU
CSB-EP015081MWU
CSB-BP015081MWU
CSB-MP015081MWU
CSB-EP015081MWU-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aplysia dactylomela L-amino-acid oxidase CSB-YP011660AML
CSB-EP011660AML
CSB-BP011660AML
CSB-MP011660AML
CSB-EP011660AML-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus N-acetyl-D-glucosamine kinase (NAGK) CSB-YP015415MRG
CSB-EP015415MRG
CSB-BP015415MRG
CSB-MP015415MRG
CSB-EP015415MRG-B
 20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式，可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中，除了表達(dá)系統(tǒng)之外，還面臨著一系列令人困惑的選擇，如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí)，你必須做出很多決定。如果你選擇的明智，你將獲得高質(zhì)量的重組蛋白，后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定，你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里，來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示，可以幫助你做出明智的決策。

第一條：重組蛋白的生產(chǎn)，在這篇文章中，我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條：如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子，它攜帶特定的基因進(jìn)入宿主細(xì)胞，并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá)，經(jīng)過多年的發(fā)展，表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示，本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條：如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用，對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題，并提供一些解決方案。除了以上文章，我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外，我們還為我們的客戶提供重組蛋白表達(dá)服務(wù)，您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問，涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等，您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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