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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Xenopus laevis Neuropilin-1 (nrp1), partial CSB-YP016091XBE
CSB-EP016091XBE
CSB-BP016091XBE
CSB-MP016091XBE
CSB-EP016091XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Haemonchus contortus Phosphoenolpyruvate carboxykinase [GTP] (PEPCK), partial CSB-YP017614HSW
CSB-EP017614HSW
CSB-BP017614HSW
CSB-MP017614HSW
CSB-EP017614HSW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xylulose kinase (xylB) CSB-YP026240KBG
CSB-EP026240KBG
CSB-BP026240KBG
CSB-MP026240KBG
CSB-EP026240KBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae DNA replication licensing factor MCM2 (MCM2), partial CSB-YP013590SVG
CSB-EP013590SVG
CSB-BP013590SVG
CSB-MP013590SVG
CSB-EP013590SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisum sativum Tubulin beta-3 chain (TUBB3) CSB-YP025323EWE
CSB-EP025323EWE
CSB-BP025323EWE
CSB-MP025323EWE
CSB-EP025323EWE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cell division control protein 6 homolog (cdc6) CSB-YP005022MEV
CSB-EP005022MEV
CSB-BP005022MEV
CSB-MP005022MEV
CSB-EP005022MEV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pomatostomus temporalis Cytochrome b (MT-CYB), partial CSB-YP015075PPM
CSB-EP015075PPM
CSB-BP015075PPM
CSB-MP015075PPM
CSB-EP015075PPM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scytalopus magellanicus Cytochrome b (MT-CYB), partial CSB-YP015075SEF
CSB-EP015075SEF
CSB-BP015075SEF
CSB-MP015075SEF
CSB-EP015075SEF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmo trutta Cytochrome c oxidase subunit 1 (mt-co1), partial CSB-YP015072SWJ1
CSB-EP015072SWJ1
CSB-BP015072SWJ1
CSB-MP015072SWJ1
CSB-EP015072SWJ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lepisosteus oculatus Cytochrome c oxidase subunit 2 (mt-co2), partial CSB-YP015073LEE1
CSB-EP015073LEE1
CSB-BP015073LEE1
CSB-MP015073LEE1
CSB-EP015073LEE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lepisosteus oculatus Cytochrome b (mt-cyb), partial CSB-YP015075LEE
CSB-EP015075LEE
CSB-BP015075LEE
CSB-MP015075LEE
CSB-EP015075LEE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bacillus subtilis GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582BRJ
CSB-EP009582BRJ
CSB-BP009582BRJ
CSB-MP009582BRJ
CSB-EP009582BRJ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Beta-galactosidase (lacA), partial CSB-YP009476DOZ
CSB-EP009476DOZ
CSB-BP009476DOZ
CSB-MP009476DOZ
CSB-EP009476DOZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila mauritiana NADH-ubiquinone oxidoreductase chain 2 (mt:ND2), partial CSB-YP015077DLT
CSB-EP015077DLT
CSB-BP015077DLT
CSB-MP015077DLT
CSB-EP015077DLT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Cytochrome c oxidase subunit 2 (COII), partial CSB-YP015073LCO1
CSB-EP015073LCO1
CSB-BP015073LCO1
CSB-MP015073LCO1
CSB-EP015073LCO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Muscarinic acetylcholine receptor M4 (chrm4), partial CSB-YP005384XBE1
CSB-EP005384XBE1
CSB-BP005384XBE1
CSB-MP005384XBE1
CSB-EP005384XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Eukaryotic translation initiation factor 3 subunit D (eif-3.D), partial CSB-YP007533CXY
CSB-EP007533CXY
CSB-BP007533CXY
CSB-MP007533CXY
CSB-EP007533CXY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Proteasome subunit beta type-5 (pts1) CSB-YP018883SXV
CSB-EP018883SXV
CSB-BP018883SXV
CSB-MP018883SXV
CSB-EP018883SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Praomys natalensis Gastrin/cholecystokinin type B receptor (CCKBR), partial CSB-YP004773PRP1
CSB-EP004773PRP1
CSB-BP004773PRP1
CSB-MP004773PRP1
CSB-EP004773PRP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acanthopagrus latus Glycoprotein hormones alpha chain (cga) CSB-YP005293AAM
CSB-EP005293AAM
CSB-BP005293AAM
CSB-MP005293AAM
CSB-EP005293AAM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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