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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Alouatta seniculus Protamine-2 (PRM2) CSB-YP018729AZU
CSB-EP018729AZU
CSB-BP018729AZU
CSB-MP018729AZU
CSB-EP018729AZU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse C-X-C chemokine receptor type 2 (Cxcr2), partial CSB-YP011673MO1
CSB-EP011673MO1
CSB-BP011673MO1
CSB-MP011673MO1
CSB-EP011673MO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Didelphis marsupialis virginiana 5-hydroxytryptamine receptor 1B (HTR1B), partial CSB-YP010882DKL1
CSB-EP010882DKL1
CSB-BP010882DKL1
CSB-MP010882DKL1
CSB-EP010882DKL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia amylovora UDP-glucose 4-epimerase (galE) CSB-YP009197EMS
CSB-EP009197EMS
CSB-BP009197EMS
CSB-MP009197EMS
CSB-EP009197EMS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica 40S ribosomal protein S20 (RPS20) CSB-YP020394OFG
CSB-EP020394OFG
CSB-BP020394OFG
CSB-MP020394OFG
CSB-EP020394OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae DNA-directed RNA polymerase III subunit RPC8 (RPC25) CSB-YP018351SVG
CSB-EP018351SVG
CSB-BP018351SVG
CSB-MP018351SVG
CSB-EP018351SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Myosin-3 (MYO3), partial CSB-YP015297SVG
CSB-EP015297SVG
CSB-BP015297SVG
CSB-MP015297SVG
CSB-EP015297SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ubiquitin carboxyl-terminal hydrolase 11 (UBP11), partial CSB-YP025698SVG
CSB-EP025698SVG
CSB-BP025698SVG
CSB-MP025698SVG
CSB-EP025698SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Mitochondrial intermembrane space import and assembly protein 40 (MIA40), partial CSB-YP005319SVG1
CSB-EP005319SVG1
CSB-BP005319SVG1
CSB-MP005319SVG1
CSB-EP005319SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae GPI ethanolamine phosphate transferase 1 (MCD4), partial CSB-YP017977SVG
CSB-EP017977SVG
CSB-BP017977SVG
CSB-MP017977SVG
CSB-EP017977SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Signal recognition particle receptor subunit beta (SRP102), partial CSB-YP022687SVG1
CSB-EP022687SVG1
CSB-BP022687SVG1
CSB-MP022687SVG1
CSB-EP022687SVG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Defensin (Def) CSB-YP006652DLU
CSB-EP006652DLU
CSB-BP006652DLU
CSB-MP006652DLU
CSB-EP006652DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Notothenia coriiceps neglecta Tubulin beta-1 chain (tubb1) CSB-YP025319NHX
CSB-EP025319NHX
CSB-BP025319NHX
CSB-MP025319NHX
CSB-EP025319NHX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine herpesvirus 5 Thymidine kinase (TK) CSB-YP023577BKA
CSB-EP023577BKA
CSB-BP023577BKA
CSB-MP023577BKA
CSB-EP023577BKA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis SPARC (sparc) CSB-YP022486XBE
CSB-EP022486XBE
CSB-BP022486XBE
CSB-MP022486XBE
CSB-EP022486XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Macaca fascicularis Sex-determining region Y protein (SRY) CSB-YP022699MOV
CSB-EP022699MOV
CSB-BP022699MOV
CSB-MP022699MOV
CSB-EP022699MOV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dictyostelium discoideum Ras-related protein Rab-11A (rab11A) CSB-YP019153DKK
CSB-EP019153DKK
CSB-BP019153DKK
CSB-MP019153DKK
CSB-EP019153DKK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Schizosaccharomyces pombe Thymidylate kinase (tmp1) CSB-YP007226SXV
CSB-EP007226SXV
CSB-BP007226SXV
CSB-MP007226SXV
CSB-EP007226SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform (Ppp2r2a) CSB-YP018564RA
CSB-EP018564RA
CSB-BP018564RA
CSB-MP018564RA
CSB-EP018564RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Adenosylhomocysteinase CSB-YP001474LOE
CSB-EP001474LOE
CSB-BP001474LOE
CSB-MP001474LOE
CSB-EP001474LOE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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