Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Brucella melitensis biotype 1 Glycine cleavage system H protein (gcvH) CSB-YP009335BMO
CSB-EP009335BMO
CSB-BP009335BMO
CSB-MP009335BMO
CSB-EP009335BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Glycine cleavage system H protein (gcvH) CSB-YP009335SKY
CSB-EP009335SKY
CSB-BP009335SKY
CSB-MP009335SKY
CSB-EP009335SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup B Glucokinase (glk) CSB-YP009319NGG
CSB-EP009319NGG
CSB-BP009319NGG
CSB-MP009319NGG
CSB-EP009319NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582SMT
CSB-EP009582SMT
CSB-BP009582SMT
CSB-MP009582SMT
CSB-EP009582SMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Delta-aminolevulinic acid dehydratase (hemB) CSB-YP001558SKX
CSB-EP001558SKX
CSB-BP001558SKX
CSB-MP001558SKX
CSB-EP001558SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhodopirellula baltica Acetyl-coenzyme A synthetase (acsA), partial CSB-YP001203RDR
CSB-EP001203RDR
CSB-BP001203RDR
CSB-MP001203RDR
CSB-EP001203RDR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Probable G-protein coupled receptor 34 (GPR34), partial CSB-YP009806EQV1
CSB-EP009806EQV1
CSB-BP009806EQV1
CSB-MP009806EQV1
CSB-EP009806EQV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Rab GDP dissociation inhibitor alpha (GDI1) CSB-YP009353EQV
CSB-EP009353EQV
CSB-BP009353EQV
CSB-MP009353EQV
CSB-EP009353EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Pyruvate dehydrogenase E1 component subunit alpha (pdhA) CSB-YP017716SKX
CSB-EP017716SKX
CSB-BP017716SKX
CSB-MP017716SKX
CSB-EP017716SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Triticum aestivum NADH-ubiquinone oxidoreductase chain 3 (ND3), partial CSB-YP015078TQN1
CSB-EP015078TQN1
CSB-BP015078TQN1
CSB-MP015078TQN1
CSB-EP015078TQN1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shigella sonnei N-glycosidase YbiA (ybiA) CSB-YP001474MVZ
CSB-EP001474MVZ
CSB-BP001474MVZ
CSB-MP001474MVZ
CSB-EP001474MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA pseudouridine synthase A (truA) CSB-YP019086CQF
CSB-EP019086CQF
CSB-BP019086CQF
CSB-MP019086CQF
CSB-EP019086CQF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Onion yellows phytoplasma Single-stranded DNA-binding protein (ssb) CSB-YP022706OBN
CSB-EP022706OBN
CSB-BP022706OBN
CSB-MP022706OBN
CSB-EP022706OBN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Geobacter sulfurreducens GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582GBK
CSB-EP009582GBK
CSB-BP009582GBK
CSB-MP009582GBK
CSB-EP009582GBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Canis latrans Hemoglobin subunit alpha (HBA) CSB-YP010147CCV
CSB-EP010147CCV
CSB-BP010147CCV
CSB-MP010147CCV
CSB-EP010147CCV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Guanylate kinase (gmk) CSB-YP010059ENV
CSB-EP010059ENV
CSB-BP010059ENV
CSB-MP010059ENV
CSB-EP010059ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Guanylate kinase (gmk) CSB-YP010059MLA
CSB-EP010059MLA
CSB-BP010059MLA
CSB-MP010059MLA
CSB-EP010059MLA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan paniscus C-C chemokine receptor type 5 (CCR5), partial CSB-YP004844EQU1
CSB-EP004844EQU1
CSB-BP004844EQU1
CSB-MP004844EQU1
CSB-EP004844EQU1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arabidopsis thaliana Cytochrome c oxidase subunit 1 (COX1), partial CSB-YP015072DOA
CSB-EP015072DOA
CSB-BP015072DOA
CSB-MP015072DOA
CSB-EP015072DOA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Agmatinase (speB) CSB-YP001447SXB
CSB-EP001447SXB
CSB-BP001447SXB
CSB-MP001447SXB
CSB-EP001447SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>