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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Bdellovibrio bacteriovorus Malate dehydrogenase (mdh) CSB-YP013623BFP
CSB-EP013623BFP
CSB-BP013623BFP
CSB-MP013623BFP
CSB-EP013623BFP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermus thermophilus Malate dehydrogenase (mdh) CSB-YP013623TNS
CSB-EP013623TNS
CSB-BP013623TNS
CSB-MP013623TNS
CSB-EP013623TNS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus furiosus Transcription initiation factor IIB (tfb) CSB-YP010001FHW
CSB-EP010001FHW
CSB-BP010001FHW
CSB-MP010001FHW
CSB-EP010001FHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrococcus furiosus Triosephosphate isomerase (tpiA) CSB-YP024102FHW
CSB-EP024102FHW
CSB-BP024102FHW
CSB-MP024102FHW
CSB-EP024102FHW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Callorhinus ursinus Nuclear receptor subfamily 1 group I member 3 (NR1I3) CSB-YP016049CBE
CSB-EP016049CBE
CSB-BP016049CBE
CSB-MP016049CBE
CSB-EP016049CBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Gossypium hirsutum 60S ribosomal protein L41 (RPL41) CSB-YP020290GHB
CSB-EP020290GHB
CSB-BP020290GHB
CSB-MP020290GHB
CSB-EP020290GHB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oryza sativa subsp. japonica 60S ribosomal protein L41 (RPL41) CSB-YP020290OFG
CSB-EP020290OFG
CSB-BP020290OFG
CSB-MP020290OFG
CSB-EP020290OFG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chicken Neuronal calcium sensor 1 (NCS1) CSB-YP008983CH
CSB-EP008983CH
CSB-BP008983CH
CSB-MP008983CH
CSB-EP008983CH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 40S ribosomal protein S13 (RPS13) CSB-YP020370WBS
CSB-EP020370WBS
CSB-BP020370WBS
CSB-MP020370WBS
CSB-EP020370WBS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Small nuclear ribonucleoprotein Sm D3 (snrpd3) CSB-YP022335XBE
CSB-EP022335XBE
CSB-BP022335XBE
CSB-MP022335XBE
CSB-EP022335XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Thymosin beta-4 (TMSB4) CSB-YP023934BO
CSB-EP023934BO
CSB-BP023934BO
CSB-MP023934BO
CSB-EP023934BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesocricetus auratus Eukaryotic peptide chain release factor subunit 1 (ETF1) CSB-YP007840MRG
CSB-EP007840MRG
CSB-BP007840MRG
CSB-MP007840MRG
CSB-EP007840MRG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Coiled-coil domain-containing protein 8 (Ccdc8) CSB-YP004744RA
CSB-EP004744RA
CSB-BP004744RA
CSB-MP004744RA
CSB-EP004744RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Elongation factor 1-alpha 1 (Eef1a1) CSB-YP007409RA
CSB-EP007409RA
CSB-BP007409RA
CSB-MP007409RA
CSB-EP007409RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Beta-crystallin B2 (Crybb2) CSB-YP006013RA
CSB-EP006013RA
CSB-BP006013RA
CSB-MP006013RA
CSB-EP006013RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pisaster ochraceus Histone H4 CSB-YP010429PKW
CSB-EP010429PKW
CSB-BP010429PKW
CSB-MP010429PKW
CSB-EP010429PKW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lytechinus pictus Histone H4 CSB-YP010429LRW
CSB-EP010429LRW
CSB-BP010429LRW
CSB-MP010429LRW
CSB-EP010429LRW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phanerochaete chrysosporium Histone H4 (H4.1) CSB-YP010429EUK
CSB-EP010429EUK
CSB-BP010429EUK
CSB-MP010429EUK
CSB-EP010429EUK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Urechis caupo Histone H4 CSB-YP010429UAQ
CSB-EP010429UAQ
CSB-BP010429UAQ
CSB-MP010429UAQ
CSB-EP010429UAQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oncorhynchus mykiss Histone H4 CSB-YP010429OEI
CSB-EP010429OEI
CSB-BP010429OEI
CSB-MP010429OEI
CSB-EP010429OEI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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