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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant tRNA pseudouridine synthase A (truA) CSB-YP019086MVZ
CSB-EP019086MVZ
CSB-BP019086MVZ
CSB-MP019086MVZ
CSB-EP019086MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA pseudouridine synthase A (truA) CSB-YP019086SMT
CSB-EP019086SMT
CSB-BP019086SMT
CSB-MP019086SMT
CSB-EP019086SMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Peptide chain release factor 1 (prfA) CSB-YP015197SKY
CSB-EP015197SKY
CSB-BP015197SKY
CSB-MP015197SKY
CSB-EP015197SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 Ribonuclease 3 (rnc) CSB-YP019809BMO
CSB-EP019809BMO
CSB-BP019809BMO
CSB-MP019809BMO
CSB-EP019809BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribonuclease 3 (rnc) CSB-YP019809SMT
CSB-EP019809SMT
CSB-BP019809SMT
CSB-MP019809SMT
CSB-EP019809SMT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup B Ribosome-recycling factor (frr) CSB-YP014941NGG
CSB-EP014941NGG
CSB-BP014941NGG
CSB-MP014941NGG
CSB-EP014941NGG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Serine hydroxymethyltransferase (glyA) CSB-YP021274TIW
CSB-EP021274TIW
CSB-BP021274TIW
CSB-MP021274TIW
CSB-EP021274TIW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Superoxide dismutase [Cu-Zn] (sodC) CSB-YP022397BMQ
CSB-EP022397BMQ
CSB-BP022397BMQ
CSB-MP022397BMQ
CSB-EP022397BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Single-stranded DNA-binding protein (ssb) CSB-YP022706NGF
CSB-EP022706NGF
CSB-BP022706NGF
CSB-MP022706NGF
CSB-EP022706NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Tropheryma whipplei Single-stranded DNA-binding protein 2 (ssb2) CSB-YP022707TIV
CSB-EP022707TIV
CSB-BP022707TIV
CSB-MP022707TIV
CSB-EP022707TIV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917BMQ
CSB-EP022917BMQ
CSB-BP022917BMQ
CSB-MP022917BMQ
CSB-EP022917BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917SUL
CSB-EP022917SUL
CSB-BP022917SUL
CSB-MP022917SUL
CSB-EP022917SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146SKX
CSB-EP019146SKX
CSB-BP019146SKX
CSB-MP019146SKX
CSB-EP019146SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146SMP
CSB-EP019146SMP
CSB-BP019146SMP
CSB-MP019146SMP
CSB-EP019146SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Triosephosphate isomerase (tpiA) CSB-YP024102SMP
CSB-EP024102SMP
CSB-BP024102SMP
CSB-MP024102SMP
CSB-EP024102SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thymidylate synthase (thyA) CSB-YP025393MVH
CSB-EP025393MVH
CSB-BP025393MVH
CSB-MP025393MVH
CSB-EP025393MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thymidylate synthase (thyA) CSB-YP025393FMW
CSB-EP025393FMW
CSB-BP025393FMW
CSB-MP025393FMW
CSB-EP025393FMW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Protoheme IX farnesyltransferase (ctaB), partial CSB-YP005824BMQ
CSB-EP005824BMQ
CSB-BP005824BMQ
CSB-MP005824BMQ
CSB-EP005824BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Isocitrate dehydrogenase [NADP] (icd) CSB-YP010990MVZ
CSB-EP010990MVZ
CSB-BP010990MVZ
CSB-MP010990MVZ
CSB-EP010990MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Isocitrate dehydrogenase [NADP] (icd) CSB-YP010990SKX
CSB-EP010990SKX
CSB-BP010990SKX
CSB-MP010990SKX
CSB-EP010990SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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