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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant 6-phosphofructokinase (pfkA) CSB-YP017822MVZ
CSB-EP017822MVZ
CSB-BP017822MVZ
CSB-MP017822MVZ
CSB-EP017822MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant 6-phosphofructokinase (pfkA) CSB-YP017822SWW
CSB-EP017822SWW
CSB-BP017822SWW
CSB-MP017822SWW
CSB-EP017822SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Inorganic pyrophosphatase (ppa) CSB-YP018410MVH
CSB-EP018410MVH
CSB-BP018410MVH
CSB-MP018410MVH
CSB-EP018410MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Inorganic pyrophosphatase (ppa) CSB-YP018410XBL
CSB-EP018410XBL
CSB-BP018410XBL
CSB-MP018410XBL
CSB-EP018410XBL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribulose-phosphate 3-epimerase (rpe) CSB-YP020097MVZ
CSB-EP020097MVZ
CSB-BP020097MVZ
CSB-MP020097MVZ
CSB-EP020097MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Hypoxanthine-guanine phosphoribosyltransferase (hpt) CSB-YP010706SKX
CSB-EP010706SKX
CSB-BP010706SKX
CSB-MP010706SKX
CSB-EP010706SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA pseudouridine synthase A (truA) CSB-YP019086EOD
CSB-EP019086EOD
CSB-BP019086EOD
CSB-MP019086EOD
CSB-EP019086EOD-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus tRNA pseudouridine synthase A (truA) CSB-YP019086SKY
CSB-EP019086SKY
CSB-BP019086SKY
CSB-MP019086SKY
CSB-EP019086SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dihydrolipoyl dehydrogenase (lpd) CSB-YP006928MVZ
CSB-EP006928MVZ
CSB-BP006928MVZ
CSB-MP006928MVZ
CSB-EP006928MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Thioredoxin reductase (trxB) CSB-YP025388SKX
CSB-EP025388SKX
CSB-BP025388SKX
CSB-MP025388SKX
CSB-EP025388SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptide chain release factor 1 (prfA) CSB-YP015197MVH
CSB-EP015197MVH
CSB-BP015197MVH
CSB-MP015197MVH
CSB-EP015197MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Peptide chain release factor 1 (prfA) CSB-YP015197SUL
CSB-EP015197SUL
CSB-BP015197SUL
CSB-MP015197SUL
CSB-EP015197SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ribonuclease 3 (rnc) CSB-YP019809SUL
CSB-EP019809SUL
CSB-BP019809SUL
CSB-MP019809SUL
CSB-EP019809SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neisseria meningitidis serogroup A / serotype 4A Ribosome-recycling factor (frr) CSB-YP014941NGF
CSB-EP014941NGF
CSB-BP014941NGF
CSB-MP014941NGF
CSB-EP014941NGF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosome-recycling factor (frr) CSB-YP014941SMH
CSB-EP014941SMH
CSB-BP014941SMH
CSB-MP014941SMH
CSB-EP014941SMH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Serine hydroxymethyltransferase (glyA) CSB-YP021274SKX
CSB-EP021274SKX
CSB-BP021274SKX
CSB-MP021274SKX
CSB-EP021274SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella melitensis biotype 1 Superoxide dismutase [Cu-Zn] (sodC) CSB-YP022397BMO
CSB-EP022397BMO
CSB-BP022397BMO
CSB-MP022397BMO
CSB-EP022397BMO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Single-stranded DNA-binding protein (ssb) CSB-YP022706SMP
CSB-EP022706SMP
CSB-BP022706SMP
CSB-MP022706SMP
CSB-EP022706SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917SXB
CSB-EP022917SXB
CSB-BP022917SXB
CSB-MP022917SXB
CSB-EP022917SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Queuine tRNA-ribosyltransferase (tgt) CSB-YP019146SKY
CSB-EP019146SKY
CSB-BP019146SKY
CSB-MP019146SKY
CSB-EP019146SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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