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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Staphylococcus aureus Lipoyl synthase (lipA) CSB-YP012927SUL
CSB-EP012927SUL
CSB-BP012927SUL
CSB-MP012927SUL
CSB-EP012927SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus tRNA dimethylallyltransferase (miaA) CSB-YP024529SKX
CSB-EP024529SKX
CSB-BP024529SKX
CSB-MP024529SKX
CSB-EP024529SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus tRNA dimethylallyltransferase (miaA) CSB-YP024529SKY
CSB-EP024529SKY
CSB-BP024529SKY
CSB-MP024529SKY
CSB-EP024529SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Molybdopterin synthase catalytic subunit (moaE) CSB-YP014706SKX
CSB-EP014706SKX
CSB-BP014706SKX
CSB-MP014706SKX
CSB-EP014706SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Molybdopterin synthase catalytic subunit (moaE) CSB-YP014706SKY
CSB-EP014706SKY
CSB-BP014706SKY
CSB-MP014706SKY
CSB-EP014706SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Nucleoside diphosphate kinase (ndk) CSB-YP015889SXB
CSB-EP015889SXB
CSB-BP015889SXB
CSB-MP015889SXB
CSB-EP015889SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Streptococcus pneumoniae Nucleoside diphosphate kinase (ndk) CSB-YP015889SMP
CSB-EP015889SMP
CSB-BP015889SMP
CSB-MP015889SMP
CSB-EP015889SMP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Oligoribonuclease (orn) CSB-YP019586SWW
CSB-EP019586SWW
CSB-BP019586SWW
CSB-MP019586SWW
CSB-EP019586SWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex (sucB), partial CSB-YP006950MVH
CSB-EP006950MVH
CSB-BP006950MVH
CSB-MP006950MVH
CSB-EP006950MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Phosphoenolpyruvate carboxykinase [GTP] (pckG), partial CSB-YP017614MVZ
CSB-EP017614MVZ
CSB-BP017614MVZ
CSB-MP017614MVZ
CSB-EP017614MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium 6-phosphofructokinase (pfkA) CSB-YP017822SXB
CSB-EP017822SXB
CSB-BP017822SXB
CSB-MP017822SXB
CSB-EP017822SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Brucella suis biovar 1 Inorganic pyrophosphatase (ppa) CSB-YP018410BMQ
CSB-EP018410BMQ
CSB-BP018410BMQ
CSB-MP018410BMQ
CSB-EP018410BMQ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Salmonella typhimurium Inorganic pyrophosphatase (ppa) CSB-YP018410SXB
CSB-EP018410SXB
CSB-BP018410SXB
CSB-MP018410SXB
CSB-EP018410SXB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Hypoxanthine-guanine phosphoribosyltransferase (hpt) CSB-YP010706SUL
CSB-EP010706SUL
CSB-BP010706SUL
CSB-MP010706SUL
CSB-EP010706SUL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Amidophosphoribosyltransferase (purF) CSB-YP018427MVH
CSB-EP018427MVH
CSB-BP018427MVH
CSB-MP018427MVH
CSB-EP018427MVH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus tRNA pseudouridine synthase A (truA) CSB-YP019086SKX
CSB-EP019086SKX
CSB-BP019086SKX
CSB-MP019086SKX
CSB-EP019086SKX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Peptide chain release factor 1 (prfA) CSB-YP015197MVZ
CSB-EP015197MVZ
CSB-BP015197MVZ
CSB-MP015197MVZ
CSB-EP015197MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribonuclease 3 (rnc) CSB-YP019809MVZ
CSB-EP019809MVZ
CSB-BP019809MVZ
CSB-MP019809MVZ
CSB-EP019809MVZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Ribonuclease 3 (rnc) CSB-YP019809SKY
CSB-EP019809SKY
CSB-BP019809SKY
CSB-MP019809SKY
CSB-EP019809SKY-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribonuclease 3 (rnc) CSB-YP019809SMU
CSB-EP019809SMU
CSB-BP019809SMU
CSB-MP019809SMU
CSB-EP019809SMU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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