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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Schizosaccharomyces pombe DNA topoisomerase 1 (top1), partial CSB-YP024056SXV
CSB-EP024056SXV
CSB-BP024056SXV
CSB-MP024056SXV
CSB-EP024056SXV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ornithine decarboxylase CSB-YP016269TQV
CSB-EP016269TQV
CSB-BP016269TQV
CSB-MP016269TQV
CSB-EP016269TQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aepyceros melampus Ribonuclease pancreatic (RNASE1) CSB-YP019789AXU
CSB-EP019789AXU
CSB-BP019789AXU
CSB-MP019789AXU
CSB-EP019789AXU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Boselaphus tragocamelus Ribonuclease pancreatic (RNASE1) CSB-YP019789BUN
CSB-EP019789BUN
CSB-BP019789BUN
CSB-MP019789BUN
CSB-EP019789BUN-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Aggrecan core protein (Acan), partial CSB-YP001130RA
CSB-BP001130RA
CSB-MP001130RA
CSB-EP001130RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Zea mays ATP synthase subunit a (ATP6), partial CSB-YP015070ZAX1
CSB-EP015070ZAX1
CSB-BP015070ZAX1
CSB-MP015070ZAX1
CSB-EP015070ZAX1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ornithine aminotransferase (CAR2) CSB-YP016242SVG
CSB-EP016242SVG
CSB-BP016242SVG
CSB-MP016242SVG
CSB-EP016242SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rhizobium meliloti 5-aminolevulinate synthase (hemA) CSB-YP001559RKU
CSB-EP001559RKU
CSB-BP001559RKU
CSB-MP001559RKU
CSB-EP001559RKU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster Casein kinase II subunit beta (CkIIbeta) CSB-YP006075DLU
CSB-EP006075DLU
CSB-BP006075DLU
CSB-MP006075DLU
CSB-EP006075DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli Glycerol-3-phosphate transporter (glpT), partial CSB-YP021610ENV
CSB-EP021610ENV
CSB-BP021610ENV
CSB-MP021610ENV
CSB-EP021610ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Stromelysin-1 (MMP3), partial CSB-YP014676HU
CSB-BP014676HU
CSB-MP014676HU
CSB-EP014676HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bradyrhizobium japonicum 5-aminolevulinate synthase (hemA) CSB-YP001559BVW
CSB-EP001559BVW
CSB-BP001559BVW
CSB-MP001559BVW
CSB-EP001559BVW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Drosophila melanogaster DNA-directed RNA polymerase II subunit RPB2 (RpII140), partial CSB-YP018328DLU
CSB-EP018328DLU
CSB-BP018328DLU
CSB-MP018328DLU
CSB-EP018328DLU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Protoheme IX farnesyltransferase (ctaB), partial CSB-YP005824PBZ
CSB-EP005824PBZ
CSB-BP005824PBZ
CSB-MP005824PBZ
CSB-EP005824PBZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 1 Thymidine kinase (TK) CSB-YP023577HJM
CSB-EP023577HJM
CSB-BP023577HJM
CSB-MP023577HJM
CSB-EP023577HJM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pig Chymotrypsin-like elastase family member 2A (CELA2A) CSB-YP005196PI
CSB-EP005196PI
CSB-BP005196PI
CSB-MP005196PI
CSB-EP005196PI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Ornithine decarboxylase (SPE1) CSB-YP016269SVG
CSB-EP016269SVG
CSB-BP016269SVG
CSB-MP016269SVG
CSB-EP016269SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. ATP synthase subunit b (atpF), partial CSB-YP002358FPZ1
CSB-EP002358FPZ1
CSB-BP002358FPZ1
CSB-MP002358FPZ1
CSB-EP002358FPZ1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Synechococcus sp. ATP synthase subunit alpha (atpA), partial CSB-YP002344FPZ
CSB-EP002344FPZ
CSB-BP002344FPZ
CSB-MP002344FPZ
CSB-EP002344FPZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Saccharomyces cerevisiae Farnesyl pyrophosphate synthase (ERG20) CSB-YP008563SVG
CSB-EP008563SVG
CSB-BP008563SVG
CSB-MP008563SVG
CSB-EP008563SVG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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