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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Beta-galactosidase (lacZ), partial CSB-YP009476KBG
CSB-EP009476KBG
CSB-BP009476KBG
CSB-MP009476KBG
CSB-EP009476KBG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Pancreatic prohormone (Ppy) CSB-YP018591RA
CSB-EP018591RA
CSB-BP018591RA
CSB-MP018591RA
CSB-EP018591RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Ig epsilon chain C region CSB-YP011153MO
CSB-EP011153MO
CSB-BP011153MO
CSB-MP011153MO
CSB-EP011153MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pan troglodytes Hemoglobin subunit zeta (HBZ1) CSB-YP010162EQV
CSB-EP010162EQV
CSB-BP010162EQV
CSB-MP010162EQV
CSB-EP010162EQV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human herpesvirus 1 Thymidine kinase (TK) CSB-YP023577HJK
CSB-EP023577HJK
CSB-BP023577HJK
CSB-MP023577HJK
CSB-EP023577HJK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Glucocorticoid receptor (Nr3c1) CSB-YP016059RA
CSB-EP016059RA
CSB-BP016059RA
CSB-MP016059RA
CSB-EP016059RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Nostoc sp. ATP synthase subunit beta (atpD) CSB-YP002350NHR
CSB-EP002350NHR
CSB-BP002350NHR
CSB-MP002350NHR
CSB-EP002350NHR-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant L-asparaginase (ansB) CSB-YP002233EGI
CSB-BP002233EGI
CSB-MP002233EGI
CSB-EP002233EGI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Escherichia coli GTPase Era (era) CSB-YP007759ENV
CSB-EP007759ENV
CSB-BP007759ENV
CSB-MP007759ENV
CSB-EP007759ENV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo pygmaeus Hemoglobin subunit alpha (HBA1) CSB-YP010147EXP
CSB-EP010147EXP
CSB-BP010147EXP
CSB-MP010147EXP
CSB-EP010147EXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse Complement C5 (C5), partial CSB-YP003995MO
CSB-EP003995MO
CSB-BP003995MO
CSB-MP003995MO
CSB-EP003995MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Protachykinin-1 (Tac1) CSB-YP023062RA
CSB-EP023062RA
CSB-BP023062RA
CSB-MP023062RA
CSB-EP023062RA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pongo pygmaeus Hemoglobin subunit theta-1 (HBQ1) CSB-YP010159EXP
CSB-EP010159EXP
CSB-BP010159EXP
CSB-MP010159EXP
CSB-EP010159EXP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lepus capensis Uteroglobin (SCGB1A1) CSB-YP020811LEX
CSB-EP020811LEX
CSB-BP020811LEX
CSB-MP020811LEX
CSB-EP020811LEX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Chloephaga melanoptera Hemoglobin subunit beta (HBB) CSB-YP010150CJC
CSB-EP010150CJC
CSB-BP010150CJC
CSB-MP010150CJC
CSB-EP010150CJC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acetyl-CoA acetyltransferase (phbA) CSB-YP001134ZAK
CSB-EP001134ZAK
CSB-BP001134ZAK
CSB-MP001134ZAK
CSB-EP001134ZAK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Nucleophosmin (npm1) CSB-YP015996XBE
CSB-EP015996XBE
CSB-BP015996XBE
CSB-MP015996XBE
CSB-EP015996XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Histone H1.0 (H1F0) CSB-YP010087HU
CSB-BP010087HU
CSB-MP010087HU
CSB-EP010087HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Gamma-crystallin B (CRYGB) CSB-YP006018HU
CSB-BP006018HU
CSB-MP006018HU
CSB-EP006018HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Spermophilus townsendii Hemoglobin subunit alpha (HBA) CSB-YP010147SJH
CSB-EP010147SJH
CSB-BP010147SJH
CSB-MP010147SJH
CSB-EP010147SJH-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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