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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Xenopus tropicalis E3 ubiquitin-protein ligase listerin (ltn1), partial CSB-YP026646XBF
CSB-EP026646XBF
CSB-BP026646XBF
CSB-MP026646XBF
CSB-EP026646XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis LisH domain-containing protein ARMC9 (armc9), partial CSB-YP002111XBF
CSB-EP002111XBF
CSB-BP002111XBF
CSB-MP002111XBF
CSB-EP002111XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Lateral signaling target protein 2 homolog (zfyve28), partial CSB-YP026476DIL
CSB-EP026476DIL
CSB-BP026476DIL
CSB-MP026476DIL
CSB-EP026476DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio CST complex subunit CTC1 (ctc1), partial CSB-YP003371DIL
CSB-EP003371DIL
CSB-BP003371DIL
CSB-MP003371DIL
CSB-EP003371DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Chromatin assembly factor 1 subunit A (chaf1a), partial CSB-YP005312DIL
CSB-EP005312DIL
CSB-BP005312DIL
CSB-MP005312DIL
CSB-EP005312DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Histone H2A deubiquitinase MYSM1 (mysm1), partial CSB-YP015382XBE
CSB-EP015382XBE
CSB-BP015382XBE
CSB-MP015382XBE
CSB-EP015382XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Aspartoacylase-2 (acy3) CSB-YP001264XBE
CSB-EP001264XBE
CSB-BP001264XBE
CSB-MP001264XBE
CSB-EP001264XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Bovine Probable methyltransferase-like protein 15 (METTL15) CSB-YP013721BO
CSB-EP013721BO
CSB-BP013721BO
CSB-MP013721BO
CSB-EP013721BO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Fat storage-inducing transmembrane protein 2 (fitm2), partial CSB-YP003679XBF1
CSB-EP003679XBF1
CSB-BP003679XBF1
CSB-MP003679XBF1
CSB-EP003679XBF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis HEAT repeat-containing protein 5A (heatr5a), partial CSB-YP010264XBF
CSB-EP010264XBF
CSB-BP010264XBF
CSB-MP010264XBF
CSB-EP010264XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Immunoglobulin superfamily member 3 (igsf3), partial CSB-YP011561XBF
CSB-EP011561XBF
CSB-BP011561XBF
CSB-MP011561XBF
CSB-EP011561XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Protein FAM69B (fam69b), partial CSB-YP008295XBF1
CSB-EP008295XBF1
CSB-BP008295XBF1
CSB-MP008295XBF1
CSB-EP008295XBF1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein FAM160B1 (fam160b1), partial CSB-YP008107XBE
CSB-EP008107XBE
CSB-BP008107XBE
CSB-MP008107XBE
CSB-EP008107XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917AVA
CSB-EP022917AVA
CSB-BP022917AVA
CSB-MP022917AVA
CSB-EP022917AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Argininosuccinate synthase (argG) CSB-YP002234AVA
CSB-EP002234AVA
CSB-BP002234AVA
CSB-MP002234AVA
CSB-EP002234AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Methionyl-tRNA formyltransferase (fmt) CSB-YP015150AVA
CSB-EP015150AVA
CSB-BP015150AVA
CSB-MP015150AVA
CSB-EP015150AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Transaldolase (tal) CSB-YP023112AVA
CSB-EP023112AVA
CSB-BP023112AVA
CSB-MP023112AVA
CSB-EP023112AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. ATP synthase subunit beta (atpD) CSB-YP002350AVA
CSB-EP002350AVA
CSB-BP002350AVA
CSB-MP002350AVA
CSB-EP002350AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. ATP synthase subunit b (atpF), partial CSB-YP002358AVA1
CSB-EP002358AVA1
CSB-BP002358AVA1
CSB-MP002358AVA1
CSB-EP002358AVA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Arthrobacter sp. Peptide chain release factor 1 (prfA) CSB-YP015197AVA
CSB-EP015197AVA
CSB-BP015197AVA
CSB-MP015197AVA
CSB-EP015197AVA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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