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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Aspergillus clavatus Deoxyhypusine hydroxylase (lia1) CSB-YP007106AVC
CSB-EP007106AVC
CSB-BP007106AVC
CSB-MP007106AVC
CSB-EP007106AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Mediator of RNA polymerase II transcription subunit 8 (med8) CSB-YP013668AVC
CSB-EP013668AVC
CSB-BP013668AVC
CSB-MP013668AVC
CSB-EP013668AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Flap endonuclease 1 (fen1) CSB-YP008585AVC
CSB-EP008585AVC
CSB-BP008585AVC
CSB-MP008585AVC
CSB-EP008585AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Molybdopterin synthase catalytic subunit (mocs2) CSB-YP014706AVC
CSB-EP014706AVC
CSB-BP014706AVC
CSB-MP014706AVC
CSB-EP014706AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Vacuolar ATPase assembly integral membrane protein VMA21 (vma21), partial CSB-YP025866AVC1
CSB-EP025866AVC1
CSB-BP025866AVC1
CSB-MP025866AVC1
CSB-EP025866AVC1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Eukaryotic translation initiation factor 3 subunit I (tif34) CSB-YP007538AVC
CSB-EP007538AVC
CSB-BP007538AVC
CSB-MP007538AVC
CSB-EP007538AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Nucleolar protein 58 (nop58), partial CSB-YP015940AVC
CSB-EP015940AVC
CSB-BP015940AVC
CSB-MP015940AVC
CSB-EP015940AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Mediator of RNA polymerase II transcription subunit 31 (soh1) CSB-YP013664AVC
CSB-EP013664AVC
CSB-BP013664AVC
CSB-MP013664AVC
CSB-EP013664AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Mediator of RNA polymerase II transcription subunit 16 (sin4), partial CSB-YP013649AVC
CSB-EP013649AVC
CSB-BP013649AVC
CSB-MP013649AVC
CSB-EP013649AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Eukaryotic translation initiation factor 3 subunit F (ACLA_026150) CSB-YP007535AVC
CSB-EP007535AVC
CSB-BP007535AVC
CSB-MP007535AVC
CSB-EP007535AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Mediator of RNA polymerase II transcription subunit 10 (nut2) CSB-YP013641AVC
CSB-EP013641AVC
CSB-BP013641AVC
CSB-MP013641AVC
CSB-EP013641AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Eukaryotic translation initiation factor 3 subunit B (prt1), partial CSB-YP007530AVC
CSB-EP007530AVC
CSB-BP007530AVC
CSB-MP007530AVC
CSB-EP007530AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Kynurenine 3-monooxygenase (bna4), partial CSB-YP012475AVC
CSB-EP012475AVC
CSB-BP012475AVC
CSB-MP012475AVC
CSB-EP012475AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus clavatus Mediator of RNA polymerase II transcription subunit 7 (med7) CSB-YP013667AVC
CSB-EP013667AVC
CSB-BP013667AVC
CSB-MP013667AVC
CSB-EP013667AVC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Mediator of RNA polymerase II transcription subunit 17 (srb4), partial CSB-YP013650NEZ
CSB-EP013650NEZ
CSB-BP013650NEZ
CSB-MP013650NEZ
CSB-EP013650NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Mediator of RNA polymerase II transcription subunit 8 (med8) CSB-YP013668NEZ
CSB-EP013668NEZ
CSB-BP013668NEZ
CSB-MP013668NEZ
CSB-EP013668NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Methylthioribose-1-phosphate isomerase (mri1) CSB-YP014799NEZ
CSB-EP014799NEZ
CSB-BP014799NEZ
CSB-MP014799NEZ
CSB-EP014799NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri DNA-directed RNA polymerase III subunit rpc3 (rpc82), partial CSB-YP018345NEZ
CSB-EP018345NEZ
CSB-BP018345NEZ
CSB-MP018345NEZ
CSB-EP018345NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Structure-specific endonuclease subunit slx1 (slx1) CSB-YP009442NEZ
CSB-EP009442NEZ
CSB-BP009442NEZ
CSB-MP009442NEZ
CSB-EP009442NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Mediator of RNA polymerase II transcription subunit 16 (sin4), partial CSB-YP013649NEZ
CSB-EP013649NEZ
CSB-BP013649NEZ
CSB-MP013649NEZ
CSB-EP013649NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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