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重組蛋白

產(chǎn)品名稱(chēng) 貨號(hào) 規(guī)格
Recombinant Danio rerio POC1 centriolar protein homolog B (poc1b) CSB-YP026040DIL
CSB-EP026040DIL
CSB-BP026040DIL
CSB-MP026040DIL
CSB-EP026040DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Neuferricin (cyb5d2) CSB-YP006312DIL
CSB-EP006312DIL
CSB-BP006312DIL
CSB-MP006312DIL
CSB-EP006312DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ateles geoffroyi Homeobox protein Hox-B1 (HOXB1) CSB-YP010660AVF
CSB-EP010660AVF
CSB-BP010660AVF
CSB-MP010660AVF
CSB-EP010660AVF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lagothrix lagotricha Homeobox protein Hox-A5 (HOXA5) CSB-YP010655LNL
CSB-EP010655LNL
CSB-BP010655LNL
CSB-MP010655LNL
CSB-EP010655LNL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lagothrix lagotricha Transcription elongation factor A protein-like 1 (TCEAL1) CSB-YP023272LNL
CSB-EP023272LNL
CSB-BP023272LNL
CSB-MP023272LNL
CSB-EP023272LNL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Maconellicoccus hirsutus 40S ribosomal protein SA CSB-YP020485MNF
CSB-EP020485MNF
CSB-BP020485MNF
CSB-MP020485MNF
CSB-EP020485MNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Maconellicoccus hirsutus 60S ribosomal protein L38 (RpL38) CSB-YP020275MNF
CSB-EP020275MNF
CSB-BP020275MNF
CSB-MP020275MNF
CSB-EP020275MNF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Equus grevyi Hexokinase-2 (HK2), partial CSB-YP010469EJB
CSB-EP010469EJB
CSB-BP010469EJB
CSB-MP010469EJB
CSB-EP010469EJB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Solea senegalensis 40S ribosomal protein S3a (rps3a) CSB-YP020444STX
CSB-EP020444STX
CSB-BP020444STX
CSB-MP020444STX
CSB-EP020444STX-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Eukaryotic translation initiation factor 3 subunit A (tif32), partial CSB-YP007543AVE
CSB-EP007543AVE
CSB-BP007543AVE
CSB-MP007543AVE
CSB-EP007543AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Aspergillus niger Eukaryotic translation initiation factor 3 subunit B (prt1), partial CSB-YP007530AVE
CSB-EP007530AVE
CSB-BP007530AVE
CSB-MP007530AVE
CSB-EP007530AVE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Elongation factor G (fusA), partial CSB-YP009373LND
CSB-EP009373LND
CSB-BP009373LND
CSB-MP009373LND
CSB-EP009373LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Protein FAM166B (fam166b) CSB-YP008121DIL
CSB-EP008121DIL
CSB-BP008121DIL
CSB-MP008121DIL
CSB-EP008121DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mouse tRNA wybutosine-synthesizing protein 5 (Tyw5) CSB-YP003860MO
CSB-EP003860MO
CSB-BP003860MO
CSB-MP003860MO
CSB-EP003860MO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis GRAM domain-containing protein 4 (gramd4), partial CSB-YP009882XBE1
CSB-EP009882XBE1
CSB-BP009882XBE1
CSB-MP009882XBE1
CSB-EP009882XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Burkholderia mallei L-threonine 3-dehydrogenase (tdh) CSB-YP023350BPP
CSB-EP023350BPP
CSB-BP023350BPP
CSB-MP023350BPP
CSB-EP023350BPP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris ATP synthase subunit delta (atpH) CSB-YP002355LND
CSB-EP002355LND
CSB-BP002355LND
CSB-MP002355LND
CSB-EP002355LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Ribosome-recycling factor (frr) CSB-YP014941LND
CSB-EP014941LND
CSB-BP014941LND
CSB-MP014941LND
CSB-EP014941LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Lactococcus lactis subsp. cremoris Elongation factor Ts (tsf) CSB-YP025124LND
CSB-EP025124LND
CSB-BP025124LND
CSB-MP025124LND
CSB-EP025124LND-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Rat Cytochrome P450 4V2 (Cyp4v2), partial CSB-YP006456RA1
CSB-EP006456RA1
CSB-BP006456RA1
CSB-MP006456RA1
CSB-EP006456RA1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶(hù)提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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