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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Neosartorya fischeri Eukaryotic translation initiation factor 3 subunit F (NFIA_016300) CSB-YP007535NEZ
CSB-EP007535NEZ
CSB-BP007535NEZ
CSB-MP007535NEZ
CSB-EP007535NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Mediator of RNA polymerase II transcription subunit 10 (nut2) CSB-YP013641NEZ
CSB-EP013641NEZ
CSB-BP013641NEZ
CSB-MP013641NEZ
CSB-EP013641NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Neosartorya fischeri Eukaryotic translation initiation factor 3 subunit A (tif32), partial CSB-YP007543NEZ
CSB-EP007543NEZ
CSB-BP007543NEZ
CSB-MP007543NEZ
CSB-EP007543NEZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus ATP synthase subunit delta (atpH1) CSB-YP002355PYF
CSB-EP002355PYF
CSB-BP002355PYF
CSB-MP002355PYF
CSB-EP002355PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917PYF
CSB-EP022917PYF
CSB-BP022917PYF
CSB-MP022917PYF
CSB-EP022917PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus Serine hydroxymethyltransferase (glyA) CSB-YP021274PYF
CSB-EP021274PYF
CSB-BP021274PYF
CSB-MP021274PYF
CSB-EP021274PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pelobacter propionicus tRNA pseudouridine synthase A (truA) CSB-YP019086PYF
CSB-EP019086PYF
CSB-BP019086PYF
CSB-MP019086PYF
CSB-EP019086PYF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Translation initiation factor IF-2 (infB), partial CSB-YP015164RIZ
CSB-EP015164RIZ
CSB-BP015164RIZ
CSB-MP015164RIZ
CSB-EP015164RIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Elongation factor G (fusA), partial CSB-YP009373RIZ
CSB-EP009373RIZ
CSB-BP009373RIZ
CSB-MP009373RIZ
CSB-EP009373RIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thymidylate kinase (tmk) CSB-YP007226RIZ
CSB-EP007226RIZ
CSB-BP007226RIZ
CSB-MP007226RIZ
CSB-EP007226RIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Ribosome-recycling factor (frr) CSB-YP014941RIZ
CSB-EP014941RIZ
CSB-BP014941RIZ
CSB-MP014941RIZ
CSB-EP014941RIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant tRNA dimethylallyltransferase (miaA) CSB-YP024529RIZ
CSB-EP024529RIZ
CSB-BP024529RIZ
CSB-MP024529RIZ
CSB-EP024529RIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant GMP synthase [glutamine-hydrolyzing] (guaA), partial CSB-YP009582RIZ
CSB-EP009582RIZ
CSB-BP009582RIZ
CSB-MP009582RIZ
CSB-EP009582RIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant ATP synthase subunit delta (atpH) CSB-YP002355RIZ
CSB-EP002355RIZ
CSB-BP002355RIZ
CSB-MP002355RIZ
CSB-EP002355RIZ-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans S-formylglutathione hydrolase (fghA) CSB-YP007823PYC
CSB-EP007823PYC
CSB-BP007823PYC
CSB-MP007823PYC
CSB-EP007823PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans Methionyl-tRNA formyltransferase (fmt) CSB-YP015150PYC
CSB-EP015150PYC
CSB-BP015150PYC
CSB-MP015150PYC
CSB-EP015150PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans Elongation factor G (fusA), partial CSB-YP009373PYC
CSB-EP009373PYC
CSB-BP009373PYC
CSB-MP009373PYC
CSB-EP009373PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans Acyl carrier protein (acpP) CSB-YP015636PYC
CSB-EP015636PYC
CSB-BP015636PYC
CSB-MP015636PYC
CSB-EP015636PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans ATP synthase subunit alpha (atpA), partial CSB-YP002344PYC
CSB-EP002344PYC
CSB-BP002344PYC
CSB-MP002344PYC
CSB-EP002344PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Paracoccus denitrificans Ribosome-recycling factor (frr) CSB-YP014941PYC
CSB-EP014941PYC
CSB-BP014941PYC
CSB-MP014941PYC
CSB-EP014941PYC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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