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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Debaryomyces hansenii ATP-dependent RNA helicase SUB2 (SUB2) CSB-YP728183DIS
CSB-EP728183DIS
CSB-BP728183DIS
CSB-MP728183DIS
CSB-EP728183DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Protein RMD9, mitochondrial (RMD9), partial CSB-YP728184DIS
CSB-EP728184DIS
CSB-BP728184DIS
CSB-MP728184DIS
CSB-EP728184DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Palmitoyltransferase PFA3 (PFA3), partial CSB-YP728185DIS1
CSB-EP728185DIS1
CSB-BP728185DIS1
CSB-MP728185DIS1
CSB-EP728185DIS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Peroxisome assembly protein 22 (PEX22), partial CSB-YP728186DIS1
CSB-EP728186DIS1
CSB-BP728186DIS1
CSB-MP728186DIS1
CSB-EP728186DIS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Mitochondrial import inner membrane translocase subunit TIM8 (TIM8) CSB-YP728187DIS
CSB-EP728187DIS
CSB-BP728187DIS
CSB-MP728187DIS
CSB-EP728187DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii DNA polymerase epsilon catalytic subunit A (POL2), partial CSB-YP728188DIS
CSB-EP728188DIS
CSB-BP728188DIS
CSB-MP728188DIS
CSB-EP728188DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Probable kinetochore protein SPC25 (SPC25) CSB-YP728189DIS
CSB-EP728189DIS
CSB-BP728189DIS
CSB-MP728189DIS
CSB-EP728189DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii 5'-3' exoribonuclease 2 (RAT1), partial CSB-YP728190DIS
CSB-EP728190DIS
CSB-BP728190DIS
CSB-MP728190DIS
CSB-EP728190DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Signal peptidase complex catalytic subunit SEC11 (SEC11), partial CSB-YP728191DIS
CSB-EP728191DIS
CSB-BP728191DIS
CSB-MP728191DIS
CSB-EP728191DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Palmitoyltransferase PFA5 (PFA5), partial CSB-YP728192DIS1
CSB-EP728192DIS1
CSB-BP728192DIS1
CSB-MP728192DIS1
CSB-EP728192DIS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Succinate dehydrogenase assembly factor 2, mitochondrial (EMI5), partial CSB-YP728193DIS
CSB-EP728193DIS
CSB-BP728193DIS
CSB-MP728193DIS
CSB-EP728193DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Diphthamide biosynthesis protein 1 (DPH1) CSB-YP728194DIS
CSB-EP728194DIS
CSB-BP728194DIS
CSB-MP728194DIS
CSB-EP728194DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii DNA-directed RNA polymerase III subunit RPC3 (RPC82), partial CSB-YP728195DIS
CSB-EP728195DIS
CSB-BP728195DIS
CSB-MP728195DIS
CSB-EP728195DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Mediator of RNA polymerase II transcription subunit 21 (SRB7) CSB-YP728196DIS
CSB-EP728196DIS
CSB-BP728196DIS
CSB-MP728196DIS
CSB-EP728196DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii 3-ketodihydrosphingosine reductase TSC10 (TSC10), partial CSB-YP728197DIS1
CSB-EP728197DIS1
CSB-BP728197DIS1
CSB-MP728197DIS1
CSB-EP728197DIS1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Probable superoxide dismutase [Mn], mitochondrial (DEHA2E01232g), partial CSB-YP728198DIS
CSB-EP728198DIS
CSB-BP728198DIS
CSB-MP728198DIS
CSB-EP728198DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii ATP-dependent RNA helicase DBP5 (DBP5) CSB-YP728199DIS
CSB-EP728199DIS
CSB-BP728199DIS
CSB-MP728199DIS
CSB-EP728199DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii Increased rDNA silencing protein 4 (IRS4), partial CSB-YP728200DIS
CSB-EP728200DIS
CSB-BP728200DIS
CSB-MP728200DIS
CSB-EP728200DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii PAB-dependent poly (A)-specific ribonuclease subunit PAN3 (PAN3), partial CSB-YP728201DIS
CSB-EP728201DIS
CSB-BP728201DIS
CSB-MP728201DIS
CSB-EP728201DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Debaryomyces hansenii FACT complex subunit POB3 (POB3), partial CSB-YP728202DIS
CSB-EP728202DIS
CSB-BP728202DIS
CSB-MP728202DIS
CSB-EP728202DIS-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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