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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Yarrowia lipolytica Altered inheritance of mitochondria protein 24, mitochondrial (AIM24) CSB-YP728263YAP
CSB-EP728263YAP
CSB-BP728263YAP
CSB-MP728263YAP
CSB-EP728263YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Myosin-1 (MYO1), partial CSB-YP728264YAP
CSB-EP728264YAP
CSB-BP728264YAP
CSB-MP728264YAP
CSB-EP728264YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Sensitive to high expression protein 9 homolog, mitochondrial (SHE9), partial CSB-YP728265YAP1
CSB-EP728265YAP1
CSB-BP728265YAP1
CSB-MP728265YAP1
CSB-EP728265YAP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica FAS1 domain-containing protein YALI0D26906g (YALI0D26906g) CSB-YP728266YAP
CSB-EP728266YAP
CSB-BP728266YAP
CSB-MP728266YAP
CSB-EP728266YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Negative regulator of the PHO system (PHO85) CSB-YP728267YAP
CSB-EP728267YAP
CSB-BP728267YAP
CSB-MP728267YAP
CSB-EP728267YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Eukaryotic translation initiation factor 3 subunit K (YALI0D23243g) CSB-YP728268YAP
CSB-EP728268YAP
CSB-BP728268YAP
CSB-MP728268YAP
CSB-EP728268YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Palmitoyltransferase ERF2 (ERF2), partial CSB-YP728269YAP1
CSB-EP728269YAP1
CSB-BP728269YAP1
CSB-MP728269YAP1
CSB-EP728269YAP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica mRNA 3'-end-processing protein RNA14 (RNA14), partial CSB-YP728270YAP
CSB-EP728270YAP
CSB-BP728270YAP
CSB-MP728270YAP
CSB-EP728270YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Putative transferase CAF17, mitochondrial (CAF17) CSB-YP728271YAP
CSB-EP728271YAP
CSB-BP728271YAP
CSB-MP728271YAP
CSB-EP728271YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica 5'-3' exoribonuclease 2 (RAT1), partial CSB-YP728272YAP
CSB-EP728272YAP
CSB-BP728272YAP
CSB-MP728272YAP
CSB-EP728272YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica NADH-cytochrome b5 reductase 2 (MCR1), partial CSB-YP728273YAP
CSB-EP728273YAP
CSB-BP728273YAP
CSB-MP728273YAP
CSB-EP728273YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica ATP-dependent RNA helicase DBP3 (DBP3), partial CSB-YP728274YAP
CSB-EP728274YAP
CSB-BP728274YAP
CSB-MP728274YAP
CSB-EP728274YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Carbamoyl-phosphate synthase arginine-specific small chain (CPA1) CSB-YP728275YAP
CSB-EP728275YAP
CSB-BP728275YAP
CSB-MP728275YAP
CSB-EP728275YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica tRNA (guanine (37)-N1)-methyltransferase (TRM5) CSB-YP728276YAP
CSB-EP728276YAP
CSB-BP728276YAP
CSB-MP728276YAP
CSB-EP728276YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica U3 small nucleolar RNA-associated protein 25 (UTP25), partial CSB-YP728277YAP
CSB-EP728277YAP
CSB-BP728277YAP
CSB-MP728277YAP
CSB-EP728277YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Mitochondrial import inner membrane translocase subunit TIM21 (TIM21), partial CSB-YP728278YAP1
CSB-EP728278YAP1
CSB-BP728278YAP1
CSB-MP728278YAP1
CSB-EP728278YAP1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica ATP-dependent RNA helicase CHL1 (CHL1), partial CSB-YP728279YAP
CSB-EP728279YAP
CSB-BP728279YAP
CSB-MP728279YAP
CSB-EP728279YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica RuvB-like helicase 1 (RVB1) CSB-YP728280YAP
CSB-EP728280YAP
CSB-BP728280YAP
CSB-MP728280YAP
CSB-EP728280YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Probable hexaprenyl pyrophosphate synthase, mitochondrial (COQ1), partial CSB-YP728281YAP
CSB-EP728281YAP
CSB-BP728281YAP
CSB-MP728281YAP
CSB-EP728281YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Yarrowia lipolytica Stress response protein NST1 (NST1), partial CSB-YP728282YAP
CSB-EP728282YAP
CSB-BP728282YAP
CSB-MP728282YAP
CSB-EP728282YAP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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