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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella sp. ATP synthase subunit beta (atpD) CSB-YP002350STT
CSB-EP002350STT
CSB-BP002350STT
CSB-MP002350STT
CSB-EP002350STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. ATP synthase subunit b (atpF), partial CSB-YP002358STT1
CSB-EP002358STT1
CSB-BP002358STT1
CSB-MP002358STT1
CSB-EP002358STT1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum TATA-box-binding protein (tbp) CSB-YP023239PZW
CSB-EP023239PZW
CSB-BP023239PZW
CSB-MP023239PZW
CSB-EP023239PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum Diphthine synthase (dphB) CSB-YP007133PZW
CSB-EP007133PZW
CSB-BP007133PZW
CSB-MP007133PZW
CSB-EP007133PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum S-adenosylmethionine decarboxylase proenzyme (speH) CSB-YP001658PZW
CSB-EP001658PZW
CSB-BP001658PZW
CSB-MP001658PZW
CSB-EP001658PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum Protein archease (Pisl_1685) CSB-YP026349PZW
CSB-EP026349PZW
CSB-BP026349PZW
CSB-MP026349PZW
CSB-EP026349PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermofilum pendens Signal recognition particle 19 kDa protein (srp19) CSB-YP022674TKC
CSB-EP022674TKC
CSB-BP022674TKC
CSB-MP022674TKC
CSB-EP022674TKC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Thermofilum pendens DNA ligase 1 (lig1), partial CSB-YP012930TKC
CSB-EP012930TKC
CSB-BP012930TKC
CSB-MP012930TKC
CSB-EP012930TKC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Xaa-Pro dipeptidase (pepQ) CSB-YP017784STK
CSB-EP017784STK
CSB-BP017784STK
CSB-MP017784STK
CSB-EP017784STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Methionyl-tRNA formyltransferase (fmt) CSB-YP015150STK
CSB-EP015150STK
CSB-BP015150STK
CSB-MP015150STK
CSB-EP015150STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Enolase-phosphatase E1 (mtnC) CSB-YP007674STK
CSB-EP007674STK
CSB-BP007674STK
CSB-MP007674STK
CSB-EP007674STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis L-threonine 3-dehydrogenase (tdh) CSB-YP023350STK
CSB-EP023350STK
CSB-BP023350STK
CSB-MP023350STK
CSB-EP023350STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Thymidylate synthase (thyA) CSB-YP025393STK
CSB-EP025393STK
CSB-BP025393STK
CSB-MP025393STK
CSB-EP025393STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Ribonuclease 3 (rnc) CSB-YP019809STK
CSB-EP019809STK
CSB-BP019809STK
CSB-MP019809STK
CSB-EP019809STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Elongation factor Ts (tsf) CSB-YP025124STK
CSB-EP025124STK
CSB-BP025124STK
CSB-MP025124STK
CSB-EP025124STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Ribosome-recycling factor (frr) CSB-YP014941STK
CSB-EP014941STK
CSB-BP014941STK
CSB-MP014941STK
CSB-EP014941STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917STK
CSB-EP022917STK
CSB-BP022917STK
CSB-MP022917STK
CSB-EP022917STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Phosphoserine aminotransferase (serC) CSB-YP018838STK
CSB-EP018838STK
CSB-BP018838STK
CSB-MP018838STK
CSB-EP018838STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Thymidylate kinase (tmk) CSB-YP007226STK
CSB-EP007226STK
CSB-BP007226STK
CSB-MP007226STK
CSB-EP007226STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Acetyl-coenzyme A synthetase (acsA), partial CSB-YP001203STK
CSB-EP001203STK
CSB-BP001203STK
CSB-MP001203STK
CSB-EP001203STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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