Your Good Partner in Biology Research

重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Shewanella baltica Translation initiation factor IF-2 (infB), partial CSB-YP015164STL
CSB-EP015164STL
CSB-BP015164STL
CSB-MP015164STL
CSB-EP015164STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Lipoyl synthase (lipA) CSB-YP012927STL
CSB-EP012927STL
CSB-BP012927STL
CSB-MP012927STL
CSB-EP012927STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella baltica Porphobilinogen deaminase (hemC) CSB-YP010524STL
CSB-EP010524STL
CSB-BP010524STL
CSB-MP010524STL
CSB-EP010524STL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Methionyl-tRNA formyltransferase (fmt) CSB-YP015150DUT
CSB-EP015150DUT
CSB-BP015150DUT
CSB-MP015150DUT
CSB-EP015150DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum ATP synthase subunit delta (atpH) CSB-YP002355DUT
CSB-EP002355DUT
CSB-BP002355DUT
CSB-MP002355DUT
CSB-EP002355DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Clostridium thermocellum Elongation factor Tu (tuf) CSB-YP025342DUT
CSB-EP025342DUT
CSB-BP025342DUT
CSB-MP025342DUT
CSB-EP025342DUT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917SUP
CSB-EP022917SUP
CSB-BP022917SUP
CSB-MP022917SUP
CSB-EP022917SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylothermus marinus Signal recognition particle 19 kDa protein (srp19) CSB-YP022674SUP
CSB-EP022674SUP
CSB-BP022674SUP
CSB-MP022674SUP
CSB-EP022674SUP-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Structure-specific endonuclease subunit SLX4 (SLX4), partial CSB-YP002843SXM
CSB-EP002843SXM
CSB-BP002843SXM
CSB-MP002843SXM
CSB-EP002843SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Eukaryotic translation initiation factor 3 subunit G (TIF35) CSB-YP007536SXM
CSB-EP007536SXM
CSB-BP007536SXM
CSB-MP007536SXM
CSB-EP007536SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Scheffersomyces stipitis Histone H2A.Z (HTZ1) CSB-YP010100SXM
CSB-EP010100SXM
CSB-BP010100SXM
CSB-MP010100SXM
CSB-EP010100SXM-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio 2-oxoglutarate and iron-dependent oxygenase domain-containing protein 2 (ogfod2) CSB-YP016310DIL
CSB-EP016310DIL
CSB-BP016310DIL
CSB-MP016310DIL
CSB-EP016310DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein odr-4 homolog (odr4), partial CSB-YP003590XBE1
CSB-EP003590XBE1
CSB-BP003590XBE1
CSB-MP003590XBE1
CSB-EP003590XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Leucine-rich repeat-containing protein 3B (lrrc3b), partial CSB-YP013137DIL1
CSB-EP013137DIL1
CSB-BP013137DIL1
CSB-MP013137DIL1
CSB-EP013137DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Mitochondrial cardiolipin hydrolase (pld6), partial CSB-YP018149DIL1
CSB-EP018149DIL1
CSB-BP018149DIL1
CSB-MP018149DIL1
CSB-EP018149DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Protein FAM162B (fam162b), partial CSB-YP008112DIL1
CSB-EP008112DIL1
CSB-BP008112DIL1
CSB-MP008112DIL1
CSB-EP008112DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Methyltransferase-like protein 20 (mettl20) CSB-YP003104DIL
CSB-EP003104DIL
CSB-BP003104DIL
CSB-MP003104DIL
CSB-EP003104DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Protein argonaute-3 (eif2c3), partial CSB-YP007521DIL
CSB-EP007521DIL
CSB-BP007521DIL
CSB-MP007521DIL
CSB-EP007521DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Solute carrier family 25 member 35 (slc25a35), partial CSB-YP021505DIL1
CSB-EP021505DIL1
CSB-BP021505DIL1
CSB-MP021505DIL1
CSB-EP021505DIL1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Danio rerio Protein AF1q (mllt11) CSB-YP014634DIL
CSB-EP014634DIL
CSB-BP014634DIL
CSB-MP014634DIL
CSB-EP014634DIL-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

Read More >>