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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Shewanella sp. Acetyl-coenzyme A synthetase (acsA), partial CSB-YP001203STT
CSB-EP001203STT
CSB-BP001203STT
CSB-MP001203STT
CSB-EP001203STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Succinyl-CoA ligase [ADP-forming] subunit beta (sucC) CSB-YP022917STT
CSB-EP022917STT
CSB-BP022917STT
CSB-MP022917STT
CSB-EP022917STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Cysteine desulfurase (iscS) CSB-YP015769STT
CSB-EP015769STT
CSB-BP015769STT
CSB-MP015769STT
CSB-EP015769STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Phosphoserine aminotransferase (serC) CSB-YP018838STT
CSB-EP018838STT
CSB-BP018838STT
CSB-MP018838STT
CSB-EP018838STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Glucose-6-phosphate isomerase (pgi), partial CSB-YP009717STT
CSB-EP009717STT
CSB-BP009717STT
CSB-MP009717STT
CSB-EP009717STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Peptide chain release factor 1 (prfA) CSB-YP015197STT
CSB-EP015197STT
CSB-BP015197STT
CSB-MP015197STT
CSB-EP015197STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. Enolase-phosphatase E1 (mtnC) CSB-YP007674STT
CSB-EP007674STT
CSB-BP007674STT
CSB-MP007674STT
CSB-EP007674STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella sp. ATP synthase subunit delta (atpH) CSB-YP002355STT
CSB-EP002355STT
CSB-BP002355STT
CSB-MP002355STT
CSB-EP002355STT-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum Signal recognition particle 19 kDa protein (srp19) CSB-YP022674PZW
CSB-EP022674PZW
CSB-BP022674PZW
CSB-MP022674PZW
CSB-EP022674PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum Flap endonuclease 1 (fen) CSB-YP008585PZW
CSB-EP008585PZW
CSB-BP008585PZW
CSB-MP008585PZW
CSB-EP008585PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum Serine hydroxymethyltransferase (glyA) CSB-YP021274PZW
CSB-EP021274PZW
CSB-BP021274PZW
CSB-MP021274PZW
CSB-EP021274PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum tRNA pseudouridine synthase A (truA) CSB-YP019086PZW
CSB-EP019086PZW
CSB-BP019086PZW
CSB-MP019086PZW
CSB-EP019086PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum Triosephosphate isomerase (tpiA) CSB-YP024102PZW
CSB-EP024102PZW
CSB-BP024102PZW
CSB-MP024102PZW
CSB-EP024102PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Pyrobaculum islandicum Transcription initiation factor IIB (tfb) CSB-YP010001PZW
CSB-EP010001PZW
CSB-BP010001PZW
CSB-MP010001PZW
CSB-EP010001PZW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Elongation factor Tu (tuf1) CSB-YP025342STK
CSB-EP025342STK
CSB-BP025342STK
CSB-MP025342STK
CSB-EP025342STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Glucose-6-phosphate isomerase (pgi), partial CSB-YP009717STK
CSB-EP009717STK
CSB-BP009717STK
CSB-MP009717STK
CSB-EP009717STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Transaldolase (tal) CSB-YP023112STK
CSB-EP023112STK
CSB-BP023112STK
CSB-MP023112STK
CSB-EP023112STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Triosephosphate isomerase (tpiA) CSB-YP024102STK
CSB-EP024102STK
CSB-BP024102STK
CSB-MP024102STK
CSB-EP024102STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis Serine hydroxymethyltransferase (glyA) CSB-YP021274STK
CSB-EP021274STK
CSB-BP021274STK
CSB-MP021274STK
CSB-EP021274STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Shewanella amazonensis tRNA pseudouridine synthase A (truA) CSB-YP019086STK
CSB-EP019086STK
CSB-BP019086STK
CSB-MP019086STK
CSB-EP019086STK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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