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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Acinetobacter sp. Histidinol-phosphate aminotransferase (hisC) CSB-YP738995AWW
CSB-EP738995AWW
CSB-BP738995AWW
CSB-MP738995AWW
CSB-EP738995AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Tryptophan synthase alpha chain (trpA) CSB-YP738996AWW
CSB-EP738996AWW
CSB-BP738996AWW
CSB-MP738996AWW
CSB-EP738996AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Nucleoside diphosphate kinase (ndk) CSB-YP738997AWW
CSB-EP738997AWW
CSB-BP738997AWW
CSB-MP738997AWW
CSB-EP738997AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Trigger factor (tig) CSB-YP738998AWW
CSB-EP738998AWW
CSB-BP738998AWW
CSB-MP738998AWW
CSB-EP738998AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. 3-isopropylmalate dehydratase small subunit (leuD) CSB-YP738999AWW
CSB-EP738999AWW
CSB-BP738999AWW
CSB-MP738999AWW
CSB-EP738999AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. DNA gyrase inhibitor YacG (yacG) CSB-YP739000AWW
CSB-EP739000AWW
CSB-BP739000AWW
CSB-MP739000AWW
CSB-EP739000AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Translation initiation factor IF-2 (infB), partial CSB-YP739001AWW
CSB-EP739001AWW
CSB-BP739001AWW
CSB-MP739001AWW
CSB-EP739001AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Putative Holliday junction resolvase (ACIAD0352) CSB-YP739002AWW
CSB-EP739002AWW
CSB-BP739002AWW
CSB-MP739002AWW
CSB-EP739002AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. 50S ribosomal protein L10 (rplJ) CSB-YP739003AWW
CSB-EP739003AWW
CSB-BP739003AWW
CSB-MP739003AWW
CSB-EP739003AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Glutamyl-Q tRNA (Asp) synthetase (gluQ) CSB-YP739004AWW
CSB-EP739004AWW
CSB-BP739004AWW
CSB-MP739004AWW
CSB-EP739004AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Transcriptional repressor NrdR (nrdR) CSB-YP739005AWW
CSB-EP739005AWW
CSB-BP739005AWW
CSB-MP739005AWW
CSB-EP739005AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. ATP synthase subunit delta (atpH) CSB-YP739006AWW
CSB-EP739006AWW
CSB-BP739006AWW
CSB-MP739006AWW
CSB-EP739006AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. L-lactate dehydrogenase [cytochrome] (lldD) CSB-YP739007AWW
CSB-EP739007AWW
CSB-BP739007AWW
CSB-MP739007AWW
CSB-EP739007AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Acinetobacter sp. Tyrosine--tRNA ligase (tyrS) CSB-YP739008AWW
CSB-EP739008AWW
CSB-BP739008AWW
CSB-MP739008AWW
CSB-EP739008AWW-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human AN1-type zinc finger protein 6 (ZFAND6) CSB-YP739009HU
CSB-EP739009HU
CSB-BP739009HU
CSB-MP739009HU
CSB-EP739009HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata ATP-dependent RNA helicase HAS1 (HAS1) CSB-YP739010CZI
CSB-EP739010CZI
CSB-BP739010CZI
CSB-MP739010CZI
CSB-EP739010CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Mitochondria fission 1 protein (FIS1), partial CSB-YP739011CZI
CSB-EP739011CZI
CSB-BP739011CZI
CSB-MP739011CZI
CSB-EP739011CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata GLC7-interacting protein 4 (GIP4), partial CSB-YP739012CZI
CSB-EP739012CZI
CSB-BP739012CZI
CSB-MP739012CZI
CSB-EP739012CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Ubiquitin-like modifier HUB1 (HUB1) CSB-YP739013CZI
CSB-EP739013CZI
CSB-BP739013CZI
CSB-MP739013CZI
CSB-EP739013CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata mRNA turnover protein 4 homolog (MRT4) CSB-YP739014CZI
CSB-EP739014CZI
CSB-BP739014CZI
CSB-MP739014CZI
CSB-EP739014CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過(guò)多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開(kāi)發(fā)過(guò)程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開(kāi)發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過(guò)多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過(guò)程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見(jiàn)問(wèn)題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問(wèn),涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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