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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Kluyveromyces lactis GDP-mannose transporter (VRG4), partial CSB-YP738413KBK
CSB-EP738413KBK
CSB-BP738413KBK
CSB-MP738413KBK
CSB-EP738413KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica ATP synthase subunit c (atpE), partial CSB-YP738414EAAB1
CSB-EP738414EAAB1
CSB-BP738414EAAB1
CSB-MP738414EAAB1
CSB-EP738414EAAB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Glucosamine--fructose-6-phosphate aminotransferase [isomerizing] (glmS), partial CSB-YP738415EAAB
CSB-EP738415EAAB
CSB-BP738415EAAB
CSB-MP738415EAAB
CSB-EP738415EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica DNA replication and repair protein recF (recF) CSB-YP738416EAAB
CSB-EP738416EAAB
CSB-BP738416EAAB
CSB-MP738416EAAB
CSB-EP738416EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica sn-glycerol-3-phosphate-binding periplasmic protein ugpB (ugpB) CSB-YP738417EAAB
CSB-EP738417EAAB
CSB-BP738417EAAB
CSB-MP738417EAAB
CSB-EP738417EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Putative agmatine deiminase (aguA) CSB-YP738418EAAB
CSB-EP738418EAAB
CSB-BP738418EAAB
CSB-MP738418EAAB
CSB-EP738418EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Met repressor (metJ) CSB-YP738419EAAB
CSB-EP738419EAAB
CSB-BP738419EAAB
CSB-MP738419EAAB
CSB-EP738419EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica ATP-dependent RNA helicase rhlB (rhlB) CSB-YP738420EAAB
CSB-EP738420EAAB
CSB-BP738420EAAB
CSB-MP738420EAAB
CSB-EP738420EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Anaerobic glycerol-3-phosphate dehydrogenase subunit B (glpB) CSB-YP738421EAAB
CSB-EP738421EAAB
CSB-BP738421EAAB
CSB-MP738421EAAB
CSB-EP738421EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Fe/S biogenesis protein NfuA (nfuA) CSB-YP738422EAAB
CSB-EP738422EAAB
CSB-BP738422EAAB
CSB-MP738422EAAB
CSB-EP738422EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Siroheme synthase 2 (cysG2) CSB-YP738423EAAB
CSB-EP738423EAAB
CSB-BP738423EAAB
CSB-MP738423EAAB
CSB-EP738423EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Serine hydroxymethyltransferase 2 (glyA2) CSB-YP738424EAAB
CSB-EP738424EAAB
CSB-BP738424EAAB
CSB-MP738424EAAB
CSB-EP738424EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica 30S ribosomal protein S10 (rpsJ) CSB-YP738425EAAB
CSB-EP738425EAAB
CSB-BP738425EAAB
CSB-MP738425EAAB
CSB-EP738425EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica 50S ribosomal protein L16 (rplP) CSB-YP738426EAAB
CSB-EP738426EAAB
CSB-BP738426EAAB
CSB-MP738426EAAB
CSB-EP738426EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica 50S ribosomal protein L18 (rplR) CSB-YP738427EAAB
CSB-EP738427EAAB
CSB-BP738427EAAB
CSB-MP738427EAAB
CSB-EP738427EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica 50S ribosomal protein L17 (rplQ) CSB-YP738428EAAB
CSB-EP738428EAAB
CSB-BP738428EAAB
CSB-MP738428EAAB
CSB-EP738428EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Isocitrate dehydrogenase kinase/phosphatase (aceK), partial CSB-YP738429EAAB
CSB-EP738429EAAB
CSB-BP738429EAAB
CSB-MP738429EAAB
CSB-EP738429EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Protein hfq (hfq) CSB-YP738430EAAB
CSB-EP738430EAAB
CSB-BP738430EAAB
CSB-MP738430EAAB
CSB-EP738430EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Phosphoglycerate kinase (pgk) CSB-YP738431EAAB
CSB-EP738431EAAB
CSB-BP738431EAAB
CSB-MP738431EAAB
CSB-EP738431EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Chaperone protein DnaK (dnaK), partial CSB-YP738432EAAB
CSB-EP738432EAAB
CSB-BP738432EAAB
CSB-MP738432EAAB
CSB-EP738432EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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