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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Propionibacterium acnes tRNA (guanine-N (1)-)-methyltransferase (trmD) CSB-YP738013PSG
CSB-EP738013PSG
CSB-BP738013PSG
CSB-MP738013PSG
CSB-EP738013PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP738014PSG
CSB-EP738014PSG
CSB-BP738014PSG
CSB-MP738014PSG
CSB-EP738014PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes Ketol-acid reductoisomerase (ilvC) CSB-YP738015PSG
CSB-EP738015PSG
CSB-BP738015PSG
CSB-MP738015PSG
CSB-EP738015PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes 50S ribosomal protein L31 (rpmE) CSB-YP738016PSG
CSB-EP738016PSG
CSB-BP738016PSG
CSB-MP738016PSG
CSB-EP738016PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes Deoxyribose-phosphate aldolase 1 (deoC1) CSB-YP738017PSG
CSB-EP738017PSG
CSB-BP738017PSG
CSB-MP738017PSG
CSB-EP738017PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes 3-dehydroquinate synthase (aroB) CSB-YP738018PSG
CSB-EP738018PSG
CSB-BP738018PSG
CSB-MP738018PSG
CSB-EP738018PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes Holliday junction ATP-dependent DNA helicase RuvA (ruvA) CSB-YP738019PSG
CSB-EP738019PSG
CSB-BP738019PSG
CSB-MP738019PSG
CSB-EP738019PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes Histidinol-phosphate aminotransferase (hisC) CSB-YP738020PSG
CSB-EP738020PSG
CSB-BP738020PSG
CSB-MP738020PSG
CSB-EP738020PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes 1,4-alpha-glucan branching enzyme GlgB (glgB), partial CSB-YP738021PSG
CSB-EP738021PSG
CSB-BP738021PSG
CSB-MP738021PSG
CSB-EP738021PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes Ribonuclease Y (rny), partial CSB-YP738022PSG
CSB-EP738022PSG
CSB-BP738022PSG
CSB-MP738022PSG
CSB-EP738022PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes Pyridoxal biosynthesis lyase pdxS (pdxS) CSB-YP738023PSG
CSB-EP738023PSG
CSB-BP738023PSG
CSB-MP738023PSG
CSB-EP738023PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes 30S ribosomal protein S20 (rpsT) CSB-YP738024PSG
CSB-EP738024PSG
CSB-BP738024PSG
CSB-MP738024PSG
CSB-EP738024PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes Nucleoside diphosphate kinase (ndk) CSB-YP738025PSG
CSB-EP738025PSG
CSB-BP738025PSG
CSB-MP738025PSG
CSB-EP738025PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes Protein CrcB homolog 2 (crcB2), partial CSB-YP738026PSG1
CSB-EP738026PSG1
CSB-BP738026PSG1
CSB-MP738026PSG1
CSB-EP738026PSG1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes Ribosomal RNA small subunit methyltransferase H (rsmH) CSB-YP738027PSG
CSB-EP738027PSG
CSB-BP738027PSG
CSB-MP738027PSG
CSB-EP738027PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes Dihydrodipicolinate synthase (dapA) CSB-YP738028PSG
CSB-EP738028PSG
CSB-BP738028PSG
CSB-MP738028PSG
CSB-EP738028PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes Peptidyl-tRNA hydrolase (pth) CSB-YP738029PSG
CSB-EP738029PSG
CSB-BP738029PSG
CSB-MP738029PSG
CSB-EP738029PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes Cobyric acid synthase (cobQ) CSB-YP738030PSG
CSB-EP738030PSG
CSB-BP738030PSG
CSB-MP738030PSG
CSB-EP738030PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes Phosphate import ATP-binding protein PstB (pstB) CSB-YP738031PSG
CSB-EP738031PSG
CSB-BP738031PSG
CSB-MP738031PSG
CSB-EP738031PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Propionibacterium acnes Thymidylate kinase (tmk) CSB-YP738032PSG
CSB-EP738032PSG
CSB-BP738032PSG
CSB-MP738032PSG
CSB-EP738032PSG-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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