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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Desulfotalea psychrophila UDP-N-acetylenolpyruvoylglucosamine reductase (murB) CSB-YP738073DAAA
CSB-EP738073DAAA
CSB-BP738073DAAA
CSB-MP738073DAAA
CSB-EP738073DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Thymidylate kinase (tmk) CSB-YP738074DAAA
CSB-EP738074DAAA
CSB-BP738074DAAA
CSB-MP738074DAAA
CSB-EP738074DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Transcriptional repressor NrdR (nrdR) CSB-YP738075DAAA
CSB-EP738075DAAA
CSB-BP738075DAAA
CSB-MP738075DAAA
CSB-EP738075DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Arginine biosynthesis bifunctional protein ArgJ (argJ) CSB-YP738076DAAA
CSB-EP738076DAAA
CSB-BP738076DAAA
CSB-MP738076DAAA
CSB-EP738076DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Peptide chain release factor 1 (prfA) CSB-YP738077DAAA
CSB-EP738077DAAA
CSB-BP738077DAAA
CSB-MP738077DAAA
CSB-EP738077DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP738078DAAA
CSB-EP738078DAAA
CSB-BP738078DAAA
CSB-MP738078DAAA
CSB-EP738078DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila tRNA pseudouridine synthase B (truB) CSB-YP738079DAAA
CSB-EP738079DAAA
CSB-BP738079DAAA
CSB-MP738079DAAA
CSB-EP738079DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila 50S ribosomal protein L9 (rplI) CSB-YP738080DAAA
CSB-EP738080DAAA
CSB-BP738080DAAA
CSB-MP738080DAAA
CSB-EP738080DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Probable nicotinate-nucleotide adenylyltransferase (nadD) CSB-YP738081DAAA
CSB-EP738081DAAA
CSB-BP738081DAAA
CSB-MP738081DAAA
CSB-EP738081DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Phosphoribosylformylglycinamidine cyclo-ligase (purM) CSB-YP738082DAAA
CSB-EP738082DAAA
CSB-BP738082DAAA
CSB-MP738082DAAA
CSB-EP738082DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila tRNA pseudouridine synthase A 2 (truA2) CSB-YP738083DAAA
CSB-EP738083DAAA
CSB-BP738083DAAA
CSB-MP738083DAAA
CSB-EP738083DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Ribosomal RNA small subunit methyltransferase J (rsmJ) CSB-YP738084DAAA
CSB-EP738084DAAA
CSB-BP738084DAAA
CSB-MP738084DAAA
CSB-EP738084DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH) CSB-YP738085DAAA
CSB-EP738085DAAA
CSB-BP738085DAAA
CSB-MP738085DAAA
CSB-EP738085DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Pantothenate synthetase (panC) CSB-YP738086DAAA
CSB-EP738086DAAA
CSB-BP738086DAAA
CSB-MP738086DAAA
CSB-EP738086DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Leucyl/phenylalanyl-tRNA--protein transferase (aat) CSB-YP738087DAAA
CSB-EP738087DAAA
CSB-BP738087DAAA
CSB-MP738087DAAA
CSB-EP738087DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Thiazole synthase (thiG) CSB-YP738088DAAA
CSB-EP738088DAAA
CSB-BP738088DAAA
CSB-MP738088DAAA
CSB-EP738088DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Chaperone protein DnaK (dnaK), partial CSB-YP738089DAAA
CSB-EP738089DAAA
CSB-BP738089DAAA
CSB-MP738089DAAA
CSB-EP738089DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila GTP cyclohydrolase folE2 (folE2) CSB-YP738090DAAA
CSB-EP738090DAAA
CSB-BP738090DAAA
CSB-MP738090DAAA
CSB-EP738090DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Adenine deaminase 2 (ade2), partial CSB-YP738091DAAA
CSB-EP738091DAAA
CSB-BP738091DAAA
CSB-MP738091DAAA
CSB-EP738091DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila N-acetyl-gamma-glutamyl-phosphate reductase (argC) CSB-YP738092DAAA
CSB-EP738092DAAA
CSB-BP738092DAAA
CSB-MP738092DAAA
CSB-EP738092DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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