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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Candida glabrata Eisosome protein 1 (EIS1), partial CSB-YP739295CZI
CSB-EP739295CZI
CSB-BP739295CZI
CSB-MP739295CZI
CSB-EP739295CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Actin-like protein ARP6 (ARP6) CSB-YP739296CZI
CSB-EP739296CZI
CSB-BP739296CZI
CSB-MP739296CZI
CSB-EP739296CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata FACT complex subunit POB3 (POB3), partial CSB-YP739297CZI
CSB-EP739297CZI
CSB-BP739297CZI
CSB-MP739297CZI
CSB-EP739297CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Histone acetyltransferase type B catalytic subunit (HAT1) CSB-YP739298CZI
CSB-EP739298CZI
CSB-BP739298CZI
CSB-MP739298CZI
CSB-EP739298CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata ATPase expression protein 3 (AEP3), partial CSB-YP739299CZI
CSB-EP739299CZI
CSB-BP739299CZI
CSB-MP739299CZI
CSB-EP739299CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Nuclear rim protein 1 (NUR1), partial CSB-YP739300CZI1
CSB-EP739300CZI1
CSB-BP739300CZI1
CSB-MP739300CZI1
CSB-EP739300CZI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata 37S ribosomal protein S9, mitochondrial (MRPS9) CSB-YP739301CZI
CSB-EP739301CZI
CSB-BP739301CZI
CSB-MP739301CZI
CSB-EP739301CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Transcription elongation factor SPT6 (SPT6), partial CSB-YP739302CZI
CSB-EP739302CZI
CSB-BP739302CZI
CSB-MP739302CZI
CSB-EP739302CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Pro-apoptotic serine protease NMA111 (NMA111), partial CSB-YP739303CZI
CSB-EP739303CZI
CSB-BP739303CZI
CSB-MP739303CZI
CSB-EP739303CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata DASH complex subunit SPC34 (SPC34) CSB-YP739304CZI
CSB-EP739304CZI
CSB-BP739304CZI
CSB-MP739304CZI
CSB-EP739304CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Glucose transport transcription regulator RGT1 (RGT1), partial CSB-YP739305CZI
CSB-EP739305CZI
CSB-BP739305CZI
CSB-MP739305CZI
CSB-EP739305CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata ATP phosphoribosyltransferase (HIS1) CSB-YP739306CZI
CSB-EP739306CZI
CSB-BP739306CZI
CSB-MP739306CZI
CSB-EP739306CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Coatomer subunit beta (SEC26), partial CSB-YP739307CZI
CSB-EP739307CZI
CSB-BP739307CZI
CSB-MP739307CZI
CSB-EP739307CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Golgi apparatus membrane protein TVP23 (TVP23), partial CSB-YP739308CZI1
CSB-EP739308CZI1
CSB-BP739308CZI1
CSB-MP739308CZI1
CSB-EP739308CZI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Cytoplasmic tRNA 2-thiolation protein 1 (NCS6) CSB-YP739309CZI
CSB-EP739309CZI
CSB-BP739309CZI
CSB-MP739309CZI
CSB-EP739309CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata L-aminoadipate-semialdehyde dehydrogenase large subunit (LYS2), partial CSB-YP739310CZI
CSB-EP739310CZI
CSB-BP739310CZI
CSB-MP739310CZI
CSB-EP739310CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Transcription activator of gluconeogenesis ERT1 (ERT1), partial CSB-YP739311CZI
CSB-EP739311CZI
CSB-BP739311CZI
CSB-MP739311CZI
CSB-EP739311CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Protein YOP1 (YOP1), partial CSB-YP739312CZI1
CSB-EP739312CZI1
CSB-BP739312CZI1
CSB-MP739312CZI1
CSB-EP739312CZI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata 40S ribosomal protein S22 (RPS22) CSB-YP739313CZI
CSB-EP739313CZI
CSB-BP739313CZI
CSB-MP739313CZI
CSB-EP739313CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Myosin-5 (MYO5), partial CSB-YP739314CZI
CSB-EP739314CZI
CSB-BP739314CZI
CSB-MP739314CZI
CSB-EP739314CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長(zhǎng)度(全長(zhǎng)或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來(lái)自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來(lái)產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來(lái)選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對(duì)其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對(duì)CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們?cè)诰€留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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