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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Leifsonia xyli subsp. xyli 50S ribosomal protein L31 type B (rpmE2) CSB-YP738053LAAC
CSB-EP738053LAAC
CSB-BP738053LAAC
CSB-MP738053LAAC
CSB-EP738053LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli UPF0042 nucleotide-binding protein Lxx11490 (Lxx11490) CSB-YP738054LAAC
CSB-EP738054LAAC
CSB-BP738054LAAC
CSB-MP738054LAAC
CSB-EP738054LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli Phosphoribosyl-AMP cyclohydrolase (hisI) CSB-YP738055LAAC
CSB-EP738055LAAC
CSB-BP738055LAAC
CSB-MP738055LAAC
CSB-EP738055LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli Aspartate carbamoyltransferase (pyrB) CSB-YP738056LAAC
CSB-EP738056LAAC
CSB-BP738056LAAC
CSB-MP738056LAAC
CSB-EP738056LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli Alanine--tRNA ligase (alaS), partial CSB-YP738057LAAC
CSB-EP738057LAAC
CSB-BP738057LAAC
CSB-MP738057LAAC
CSB-EP738057LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli Threonine--tRNA ligase (thrS), partial CSB-YP738058LAAC
CSB-EP738058LAAC
CSB-BP738058LAAC
CSB-MP738058LAAC
CSB-EP738058LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli Thymidylate synthase (thyA) CSB-YP738059LAAC
CSB-EP738059LAAC
CSB-BP738059LAAC
CSB-MP738059LAAC
CSB-EP738059LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli 3-isopropylmalate dehydratase large subunit (leuC) CSB-YP738060LAAC
CSB-EP738060LAAC
CSB-BP738060LAAC
CSB-MP738060LAAC
CSB-EP738060LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli Gamma-glutamyl phosphate reductase (proA) CSB-YP738061LAAC
CSB-EP738061LAAC
CSB-BP738061LAAC
CSB-MP738061LAAC
CSB-EP738061LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli ATP-dependent Clp protease proteolytic subunit 2 (clpP2) CSB-YP738062LAAC
CSB-EP738062LAAC
CSB-BP738062LAAC
CSB-MP738062LAAC
CSB-EP738062LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli ATP synthase subunit alpha (atpA), partial CSB-YP738063LAAC
CSB-EP738063LAAC
CSB-BP738063LAAC
CSB-MP738063LAAC
CSB-EP738063LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli Carbon storage regulator homolog (csrA) CSB-YP738064LAAC
CSB-EP738064LAAC
CSB-BP738064LAAC
CSB-MP738064LAAC
CSB-EP738064LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli Phenylalanine--tRNA ligase alpha subunit (pheS) CSB-YP738065LAAC
CSB-EP738065LAAC
CSB-BP738065LAAC
CSB-MP738065LAAC
CSB-EP738065LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli Tryptophan--tRNA ligase (trpS) CSB-YP738066LAAC
CSB-EP738066LAAC
CSB-BP738066LAAC
CSB-MP738066LAAC
CSB-EP738066LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli 50S ribosomal protein L7/L12 (rplL) CSB-YP738067LAAC
CSB-EP738067LAAC
CSB-BP738067LAAC
CSB-MP738067LAAC
CSB-EP738067LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli Uracil phosphoribosyltransferase (upp) CSB-YP738068LAAC
CSB-EP738068LAAC
CSB-BP738068LAAC
CSB-MP738068LAAC
CSB-EP738068LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Leifsonia xyli subsp. xyli Mannitol-1-phosphate 5-dehydrogenase (mtlD) CSB-YP738069LAAC
CSB-EP738069LAAC
CSB-BP738069LAAC
CSB-MP738069LAAC
CSB-EP738069LAAC-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Human Uncharacterized protein KIAA0240 (KIAA0240), partial CSB-YP738070HU
CSB-EP738070HU
CSB-BP738070HU
CSB-MP738070HU
CSB-EP738070HU-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila UPF0061 protein DP3021 (DP3021) CSB-YP738071DAAA
CSB-EP738071DAAA
CSB-BP738071DAAA
CSB-MP738071DAAA
CSB-EP738071DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Desulfotalea psychrophila Ribosomal RNA large subunit methyltransferase E (rlmE) CSB-YP738072DAAA
CSB-EP738072DAAA
CSB-BP738072DAAA
CSB-MP738072DAAA
CSB-EP738072DAAA-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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