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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Kluyveromyces lactis Protein transport protein SEC22 (SEC22), partial CSB-YP738333KBK
CSB-EP738333KBK
CSB-BP738333KBK
CSB-MP738333KBK
CSB-EP738333KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Genetic interactor of prohibitin 5, mitochondrial (GEP5) CSB-YP738334KBK
CSB-EP738334KBK
CSB-BP738334KBK
CSB-MP738334KBK
CSB-EP738334KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis 37S ribosomal protein S10, mitochondrial (RSM10) CSB-YP738335KBK
CSB-EP738335KBK
CSB-BP738335KBK
CSB-MP738335KBK
CSB-EP738335KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Pre-mRNA-splicing factor ISY1 (ISY1) CSB-YP738336KBK
CSB-EP738336KBK
CSB-BP738336KBK
CSB-MP738336KBK
CSB-EP738336KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Maintenance of mitochondrial morphology protein 1 (MMM1), partial CSB-YP738337KBK
CSB-EP738337KBK
CSB-BP738337KBK
CSB-MP738337KBK
CSB-EP738337KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis ATP-dependent RNA helicase DBP7 (DBP7), partial CSB-YP738338KBK
CSB-EP738338KBK
CSB-BP738338KBK
CSB-MP738338KBK
CSB-EP738338KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Protein BUR2 (BUR2) CSB-YP738339KBK
CSB-EP738339KBK
CSB-BP738339KBK
CSB-MP738339KBK
CSB-EP738339KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Pre-mRNA-splicing factor PRP46 (PRP46) CSB-YP738340KBK
CSB-EP738340KBK
CSB-BP738340KBK
CSB-MP738340KBK
CSB-EP738340KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Ribosome biogenesis protein NSA1 (NSA1) CSB-YP738341KBK
CSB-EP738341KBK
CSB-BP738341KBK
CSB-MP738341KBK
CSB-EP738341KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Mitochondrial distribution and morphology protein 10 (MDM10) CSB-YP738342KBK
CSB-EP738342KBK
CSB-BP738342KBK
CSB-MP738342KBK
CSB-EP738342KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Outer spore wall protein 5 (OSW5), partial CSB-YP738343KBK
CSB-EP738343KBK
CSB-BP738343KBK
CSB-MP738343KBK
CSB-EP738343KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Spindle pole component BBP1 (BBP1) CSB-YP738344KBK
CSB-EP738344KBK
CSB-BP738344KBK
CSB-MP738344KBK
CSB-EP738344KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Protein transport protein SEC31 (SEC31), partial CSB-YP738345KBK
CSB-EP738345KBK
CSB-BP738345KBK
CSB-MP738345KBK
CSB-EP738345KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Vacuolar membrane protein KLLA0F03465g (KLLA0F03465g), partial CSB-YP738346KBK1
CSB-EP738346KBK1
CSB-BP738346KBK1
CSB-MP738346KBK1
CSB-EP738346KBK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis GLC7-interacting protein 4 (GIP4), partial CSB-YP738347KBK
CSB-EP738347KBK
CSB-BP738347KBK
CSB-MP738347KBK
CSB-EP738347KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis GPI ethanolamine phosphate transferase 2 (LAS21), partial CSB-YP738348KBK
CSB-EP738348KBK
CSB-BP738348KBK
CSB-MP738348KBK
CSB-EP738348KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Imidazoleglycerol-phosphate dehydratase (HIS3) CSB-YP738349KBK
CSB-EP738349KBK
CSB-BP738349KBK
CSB-MP738349KBK
CSB-EP738349KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Translocation protein SEC62 (SEC62), partial CSB-YP738350KBK1
CSB-EP738350KBK1
CSB-BP738350KBK1
CSB-MP738350KBK1
CSB-EP738350KBK1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis ATP-dependent RNA helicase SUB2 (SUB2) CSB-YP738351KBK
CSB-EP738351KBK
CSB-BP738351KBK
CSB-MP738351KBK
CSB-EP738351KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Kluyveromyces lactis Biogenesis of lysosome-related organelles complex 1 subunit CNL1 (CLN1) CSB-YP738352KBK
CSB-EP738352KBK
CSB-BP738352KBK
CSB-MP738352KBK
CSB-EP738352KBK-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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