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重組蛋白

產(chǎn)品名稱 貨號(hào) 規(guī)格
Recombinant Candida glabrata rRNA 2'-O-methyltransferase fibrillarin (NOP1) CSB-YP739315CZI
CSB-EP739315CZI
CSB-BP739315CZI
CSB-MP739315CZI
CSB-EP739315CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Ribosome biogenesis protein SLX9 (SLX9) CSB-YP739316CZI
CSB-EP739316CZI
CSB-BP739316CZI
CSB-MP739316CZI
CSB-EP739316CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Sorting nexin MVP1 (MVP1), partial CSB-YP739317CZI
CSB-EP739317CZI
CSB-BP739317CZI
CSB-MP739317CZI
CSB-EP739317CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Pre-rRNA-processing protein IPI3 (IPI3), partial CSB-YP739318CZI
CSB-EP739318CZI
CSB-BP739318CZI
CSB-MP739318CZI
CSB-EP739318CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Histone-lysine N-methyltransferase, H3 lysine-79 specific (DOT1), partial CSB-YP739319CZI
CSB-EP739319CZI
CSB-BP739319CZI
CSB-MP739319CZI
CSB-EP739319CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Methylated-DNA--protein-cysteine methyltransferase (MGT1) CSB-YP739320CZI
CSB-EP739320CZI
CSB-BP739320CZI
CSB-MP739320CZI
CSB-EP739320CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Protein transport protein SEC13-1 (SEC131) CSB-YP739321CZI
CSB-EP739321CZI
CSB-BP739321CZI
CSB-MP739321CZI
CSB-EP739321CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata DNA polymerase epsilon catalytic subunit A (POL2), partial CSB-YP739322CZI
CSB-EP739322CZI
CSB-BP739322CZI
CSB-MP739322CZI
CSB-EP739322CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Peroxisome assembly protein 22 (PEX22), partial CSB-YP739323CZI
CSB-EP739323CZI
CSB-BP739323CZI
CSB-MP739323CZI
CSB-EP739323CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Signal recognition particle SEC65 subunit (SEC65) CSB-YP739324CZI
CSB-EP739324CZI
CSB-BP739324CZI
CSB-MP739324CZI
CSB-EP739324CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Actin cytoskeleton-regulatory complex protein END3 (END3) CSB-YP739325CZI
CSB-EP739325CZI
CSB-BP739325CZI
CSB-MP739325CZI
CSB-EP739325CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Postreplication repair E3 ubiquitin-protein ligase RAD18 (RAD18) CSB-YP739326CZI
CSB-EP739326CZI
CSB-BP739326CZI
CSB-MP739326CZI
CSB-EP739326CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata 1- (5-phosphoribosyl)-5-[ (5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (HIS6) CSB-YP739327CZI
CSB-EP739327CZI
CSB-BP739327CZI
CSB-MP739327CZI
CSB-EP739327CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Glyceraldehyde-3-phosphate dehydrogenase 1 (GPD1) CSB-YP739328CZI
CSB-EP739328CZI
CSB-BP739328CZI
CSB-MP739328CZI
CSB-EP739328CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Pre-mRNA-splicing factor SYF2 (SYF2) CSB-YP739329CZI
CSB-EP739329CZI
CSB-BP739329CZI
CSB-MP739329CZI
CSB-EP739329CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Required for respiratory growth protein 7, mitochondrial (RRG7) CSB-YP739330CZI
CSB-EP739330CZI
CSB-BP739330CZI
CSB-MP739330CZI
CSB-EP739330CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Serine hydroxymethyltransferase, mitochondrial (SHM1) CSB-YP739331CZI
CSB-EP739331CZI
CSB-BP739331CZI
CSB-MP739331CZI
CSB-EP739331CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata DNA-directed RNA polymerases I, II, and III subunit RPABC1 (RPB5) CSB-YP739332CZI
CSB-EP739332CZI
CSB-BP739332CZI
CSB-MP739332CZI
CSB-EP739332CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Serine/threonine-protein kinase HAL5 (HAL5), partial CSB-YP739333CZI
CSB-EP739333CZI
CSB-BP739333CZI
CSB-MP739333CZI
CSB-EP739333CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Transcription factor NRM1 (NRM1) CSB-YP739334CZI
CSB-EP739334CZI
CSB-BP739334CZI
CSB-MP739334CZI
CSB-EP739334CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時(shí),你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯(cuò)誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個(gè)有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個(gè)有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個(gè)主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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