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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Erwinia carotovora subsp. atroseptica NADH-quinone oxidoreductase subunit H (nuoH), partial CSB-YP738453EAAB
CSB-EP738453EAAB
CSB-BP738453EAAB
CSB-MP738453EAAB
CSB-EP738453EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Glutathione import ATP-binding protein GsiA (gsiA), partial CSB-YP738454EAAB
CSB-EP738454EAAB
CSB-BP738454EAAB
CSB-MP738454EAAB
CSB-EP738454EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica 8-amino-7-oxononanoate synthase (bioF) CSB-YP738455EAAB
CSB-EP738455EAAB
CSB-BP738455EAAB
CSB-MP738455EAAB
CSB-EP738455EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Nucleoid-associated protein ECA2747 (ECA2747) CSB-YP738456EAAB
CSB-EP738456EAAB
CSB-BP738456EAAB
CSB-MP738456EAAB
CSB-EP738456EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Putative transport protein ECA2683 (ECA2683), partial CSB-YP738457EAAB
CSB-EP738457EAAB
CSB-BP738457EAAB
CSB-MP738457EAAB
CSB-EP738457EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Outer-membrane lipoprotein carrier protein (lolA) CSB-YP738458EAAB
CSB-EP738458EAAB
CSB-BP738458EAAB
CSB-MP738458EAAB
CSB-EP738458EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Imidazole glycerol phosphate synthase subunit HisH (hisH) CSB-YP738459EAAB
CSB-EP738459EAAB
CSB-BP738459EAAB
CSB-MP738459EAAB
CSB-EP738459EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Lipid A export ATP-binding/permease protein MsbA (msbA), partial CSB-YP738460EAAB1
CSB-EP738460EAAB1
CSB-BP738460EAAB1
CSB-MP738460EAAB1
CSB-EP738460EAAB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Asparagine--tRNA ligase (asnS) CSB-YP738461EAAB
CSB-EP738461EAAB
CSB-BP738461EAAB
CSB-MP738461EAAB
CSB-EP738461EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Copper homeostasis protein CutC (cutC) CSB-YP738462EAAB
CSB-EP738462EAAB
CSB-BP738462EAAB
CSB-MP738462EAAB
CSB-EP738462EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Holliday junction ATP-dependent DNA helicase RuvB (ruvB) CSB-YP738463EAAB
CSB-EP738463EAAB
CSB-BP738463EAAB
CSB-MP738463EAAB
CSB-EP738463EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Protease HtpX (htpX), partial CSB-YP738464EAAB1
CSB-EP738464EAAB1
CSB-BP738464EAAB1
CSB-MP738464EAAB1
CSB-EP738464EAAB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica NH (3)-dependent NAD (+) synthetase (nadE) CSB-YP738465EAAB
CSB-EP738465EAAB
CSB-BP738465EAAB
CSB-MP738465EAAB
CSB-EP738465EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Disulfide bond formation protein B (dsbB), partial CSB-YP738466EAAB1
CSB-EP738466EAAB1
CSB-BP738466EAAB1
CSB-MP738466EAAB1
CSB-EP738466EAAB1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica UPF0263 protein ECA2319 (ECA2319) CSB-YP738467EAAB
CSB-EP738467EAAB
CSB-BP738467EAAB
CSB-MP738467EAAB
CSB-EP738467EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Electron transport complex protein RnfD (rnfD), partial CSB-YP738468EAAB
CSB-EP738468EAAB
CSB-BP738468EAAB
CSB-MP738468EAAB
CSB-EP738468EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica UPF0482 protein ECA2253 (ECA2253) CSB-YP738469EAAB
CSB-EP738469EAAB
CSB-BP738469EAAB
CSB-MP738469EAAB
CSB-EP738469EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Peptidyl-tRNA hydrolase (pth) CSB-YP738470EAAB
CSB-EP738470EAAB
CSB-BP738470EAAB
CSB-MP738470EAAB
CSB-EP738470EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Pyridoxamine kinase (pdxY) CSB-YP738471EAAB
CSB-EP738471EAAB
CSB-BP738471EAAB
CSB-MP738471EAAB
CSB-EP738471EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Erwinia carotovora subsp. atroseptica Catalase-peroxidase (katG), partial CSB-YP738472EAAB
CSB-EP738472EAAB
CSB-BP738472EAAB
CSB-MP738472EAAB
CSB-EP738472EAAB-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達載體的選擇、表達蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達載體?

表達載體是一種DNA分子,它攜帶特定的基因進入宿主細胞,并利用細胞的蛋白質(zhì)合成機制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達載體可使外源基因在宿主細胞中高效表達,經(jīng)過多年的發(fā)展,表達載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達載體。

第三條:如何表達具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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