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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Candida glabrata Mediator of RNA polymerase II transcription subunit 31 (SOH1) CSB-YP739475CZI
CSB-EP739475CZI
CSB-BP739475CZI
CSB-MP739475CZI
CSB-EP739475CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Mitochondrial import inner membrane translocase subunit TIM13 (TIM13) CSB-YP739476CZI
CSB-EP739476CZI
CSB-BP739476CZI
CSB-MP739476CZI
CSB-EP739476CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Autophagy-related protein 11 (ATG11), partial CSB-YP739477CZI
CSB-EP739477CZI
CSB-BP739477CZI
CSB-MP739477CZI
CSB-EP739477CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Serine/threonine-protein phosphatase 2A activator 1 (RRD1) CSB-YP739478CZI
CSB-EP739478CZI
CSB-BP739478CZI
CSB-MP739478CZI
CSB-EP739478CZI-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Candida glabrata Altered inheritance of mitochondria protein 43, mitochondrial (AIM43), partial CSB-YP739479CZI1
CSB-EP739479CZI1
CSB-BP739479CZI1
CSB-MP739479CZI1
CSB-EP739479CZI1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesoplasma florum DNA-directed RNA polymerase subunit beta' (rpoC), partial CSB-YP739480MEO
CSB-EP739480MEO
CSB-BP739480MEO
CSB-MP739480MEO
CSB-EP739480MEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesoplasma florum 50S ribosomal protein L19 (rplS) CSB-YP739481MEO
CSB-EP739481MEO
CSB-BP739481MEO
CSB-MP739481MEO
CSB-EP739481MEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesoplasma florum 50S ribosomal protein L13 (rplM) CSB-YP739482MEO
CSB-EP739482MEO
CSB-BP739482MEO
CSB-MP739482MEO
CSB-EP739482MEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesoplasma florum 50S ribosomal protein L27 (rpmA) CSB-YP739483MEO
CSB-EP739483MEO
CSB-BP739483MEO
CSB-MP739483MEO
CSB-EP739483MEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesoplasma florum Phenylalanine--tRNA ligase beta subunit (pheT), partial CSB-YP739484MEO
CSB-EP739484MEO
CSB-BP739484MEO
CSB-MP739484MEO
CSB-EP739484MEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesoplasma florum Probable GTP-binding protein EngB (engB) CSB-YP739485MEO
CSB-EP739485MEO
CSB-BP739485MEO
CSB-MP739485MEO
CSB-EP739485MEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesoplasma florum Ribosome maturation factor RimP (rimP) CSB-YP739486MEO
CSB-EP739486MEO
CSB-BP739486MEO
CSB-MP739486MEO
CSB-EP739486MEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesoplasma florum Phosphate import ATP-binding protein PstB (pstB) CSB-YP739487MEO
CSB-EP739487MEO
CSB-BP739487MEO
CSB-MP739487MEO
CSB-EP739487MEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesoplasma florum Bifunctional protein FolD (folD) CSB-YP739488MEO
CSB-EP739488MEO
CSB-BP739488MEO
CSB-MP739488MEO
CSB-EP739488MEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesoplasma florum 50S ribosomal protein L18 (rplR) CSB-YP739489MEO
CSB-EP739489MEO
CSB-BP739489MEO
CSB-MP739489MEO
CSB-EP739489MEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesoplasma florum 50S ribosomal protein L16 (rplP) CSB-YP739490MEO
CSB-EP739490MEO
CSB-BP739490MEO
CSB-MP739490MEO
CSB-EP739490MEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesoplasma florum 50S ribosomal protein L23 (rplW) CSB-YP739491MEO
CSB-EP739491MEO
CSB-BP739491MEO
CSB-MP739491MEO
CSB-EP739491MEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesoplasma florum ATP synthase subunit c (atpE), partial CSB-YP739492MEO1
CSB-EP739492MEO1
CSB-BP739492MEO1
CSB-MP739492MEO1
CSB-EP739492MEO1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesoplasma florum FMN-dependent NADH-azoreductase 2 (azoR2) CSB-YP739493MEO
CSB-EP739493MEO
CSB-BP739493MEO
CSB-MP739493MEO
CSB-EP739493MEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Mesoplasma florum Ribosomal RNA small subunit methyltransferase A (rsmA) CSB-YP739494MEO
CSB-EP739494MEO
CSB-BP739494MEO
CSB-MP739494MEO
CSB-EP739494MEO-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實(shí)驗(yàn)更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗(yàn)總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點(diǎn)擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價(jià)格、交貨、質(zhì)量等,您可以點(diǎn)擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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