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重組蛋白

產(chǎn)品名稱 貨號 規(guī)格
Recombinant Staphylococcus aureus Xanthine phosphoribosyltransferase (xpt) CSB-YP740075SKV
CSB-EP740075SKV
CSB-BP740075SKV
CSB-MP740075SKV
CSB-EP740075SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus UPF0324 membrane protein SAR0338 (SAR0338), partial CSB-YP740076SKV
CSB-EP740076SKV
CSB-BP740076SKV
CSB-MP740076SKV
CSB-EP740076SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Putative ribose uptake protein rbsU (rbsU), partial CSB-YP740077SKV
CSB-EP740077SKV
CSB-BP740077SKV
CSB-MP740077SKV
CSB-EP740077SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Pyruvate formate-lyase-activating enzyme (pflA) CSB-YP740078SKV
CSB-EP740078SKV
CSB-BP740078SKV
CSB-MP740078SKV
CSB-EP740078SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Acetylglutamate kinase (argB) CSB-YP740079SKV
CSB-EP740079SKV
CSB-BP740079SKV
CSB-MP740079SKV
CSB-EP740079SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus HTH-type transcriptional regulator sarS (sarS) CSB-YP740080SKV
CSB-EP740080SKV
CSB-BP740080SKV
CSB-MP740080SKV
CSB-EP740080SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus Sensor protein kinase walK (walK), partial CSB-YP740081SKV1
CSB-EP740081SKV1
CSB-BP740081SKV1
CSB-MP740081SKV1
CSB-EP740081SKV1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Staphylococcus aureus DNA polymerase III subunit beta (dnaN) CSB-YP740082SKV
CSB-EP740082SKV
CSB-BP740082SKV
CSB-MP740082SKV
CSB-EP740082SKV-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Ubiquitin domain-containing protein 1 (ubtd1) CSB-YP740083XBF
CSB-EP740083XBF
CSB-BP740083XBF
CSB-MP740083XBF
CSB-EP740083XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Growth hormone-regulated TBC protein 1 (grtp1) CSB-YP740084XBF
CSB-EP740084XBF
CSB-BP740084XBF
CSB-MP740084XBF
CSB-EP740084XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Protein phosphatase 1 regulatory subunit 11 (ppp1r11) CSB-YP740085XBF
CSB-EP740085XBF
CSB-BP740085XBF
CSB-MP740085XBF
CSB-EP740085XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Fragile X mental retardation protein 1 homolog (fmr1), partial CSB-YP740086XBF
CSB-EP740086XBF
CSB-BP740086XBF
CSB-MP740086XBF
CSB-EP740086XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus tropicalis Leucine-rich repeat-containing protein 28 (lrrc28) CSB-YP740087XBF
CSB-EP740087XBF
CSB-BP740087XBF
CSB-MP740087XBF
CSB-EP740087XBF-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis PDZ and LIM domain protein 3 (pdlim3) CSB-YP740088XBE
CSB-EP740088XBE
CSB-BP740088XBE
CSB-MP740088XBE
CSB-EP740088XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis NEDD4 family-interacting protein 1 (ndfip1), partial CSB-YP740089XBE1
CSB-EP740089XBE1
CSB-BP740089XBE1
CSB-MP740089XBE1
CSB-EP740089XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Probable E3 ubiquitin-protein ligase makorin-1 (mkrn1) CSB-YP740090XBE
CSB-EP740090XBE
CSB-BP740090XBE
CSB-MP740090XBE
CSB-EP740090XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Protein tweety homolog 1-B (ttyh1-b), partial CSB-YP740091XBE1
CSB-EP740091XBE1
CSB-BP740091XBE1
CSB-MP740091XBE1
CSB-EP740091XBE1-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Inhibitor of nuclear factor kappa-B kinase subunit alpha (chuk), partial CSB-YP740092XBE
CSB-EP740092XBE
CSB-BP740092XBE
CSB-MP740092XBE
CSB-EP740092XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Histone H2A.V (h2afv) CSB-YP740093XBE
CSB-EP740093XBE
CSB-BP740093XBE
CSB-MP740093XBE
CSB-EP740093XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)
Recombinant Xenopus laevis Sister chromatid cohesion protein DCC1 (dscc1) CSB-YP740094XBE
CSB-EP740094XBE
CSB-BP740094XBE
CSB-MP740094XBE
CSB-EP740094XBE-B
20μg/100μg/1mg(1mg*1 or 500ug*2)

有用資源

什么是重組蛋白?

重組蛋白是一種可操縱的蛋白質(zhì)形式,可以通過多種方式產(chǎn)生大量蛋白質(zhì)、修改基因序列和制造有用的商業(yè)產(chǎn)品。重組蛋白的技術(shù)資源在重組蛋白的開發(fā)過程中,除了表達(dá)系統(tǒng)之外,還面臨著一系列令人困惑的選擇,如表達(dá)載體的選擇、表達(dá)蛋白的長度(全長或部分)、是否帶標(biāo)簽等。在生產(chǎn)重組蛋白時,你必須做出很多決定。如果你選擇的明智,你將獲得高質(zhì)量的重組蛋白,后續(xù)的實驗更有可能成功。但是如果你做了錯誤的決定,你可能得不到你所需要的重組蛋白或者重組蛋白的質(zhì)量和純度不符合要求。在這里,來自CUSABIO的蛋白表達(dá)工程師根據(jù)他們開發(fā)重組蛋白的經(jīng)驗總結(jié)出了幾個有用的提示,可以幫助你做出明智的決策。

第一條:重組蛋白的生產(chǎn),在這篇文章中,我們旨在闡明重組蛋白的定義以及如何生產(chǎn)重組蛋白。

第二條:如何選擇合適的表達(dá)載體?

表達(dá)載體是一種DNA分子,它攜帶特定的基因進(jìn)入宿主細(xì)胞,并利用細(xì)胞的蛋白質(zhì)合成機(jī)制來產(chǎn)生由該基因編碼的蛋白質(zhì)。表達(dá)載體可使外源基因在宿主細(xì)胞中高效表達(dá),經(jīng)過多年的發(fā)展,表達(dá)載體已成為一種成熟而受歡迎的生物技術(shù)。在重組蛋白生產(chǎn)過程中是一種基本成分。正如標(biāo)題所示,本文主要總結(jié)了幾個有用的技巧來選擇合適的表達(dá)載體。

第三條:如何表達(dá)具有生物活性的蛋白質(zhì)?

根據(jù)重組蛋白的不同應(yīng)用,對其生物活性有不同的要求。但是我們?nèi)绾未_保蛋白質(zhì)的產(chǎn)生具有生物活性呢?本文將圍繞這個主題,并提供一些解決方案。除了以上文章,我們還收集了一些重組蛋白的常見問題。您可以單擊以下鏈接查看。http://m.cstxtech.com/technology-91.html。此外,我們還為我們的客戶提供重組蛋白表達(dá)服務(wù),您可以點擊這里獲取更多信息。注:如果您對CUSABIO的蛋白質(zhì)有任何疑問,涉及到蛋白質(zhì)的價格、交貨、質(zhì)量等,您可以點擊橙色按鈕為我們在線留言。

引用資料

Recombinant Severe acute respiratory syndrome coronavirus 2 RNA-dependent RNA polymerase(NSP12) cited in "SARS-CoV-2 hijacks cellular kinase CDK2 to promote viral RNA synthesis". Signal transduction and targeted therapy, 2023

Recombinant Mouse Mixed lineage kinase domain-like protein(Mlkl) cited in "OASL phase condensation induces amyloid-like fibrillation of RIPK3 to promote virus-induced necroptosis". Nature cell biology, 2023

Recombinant Mouse Proto-oncogene c-Fos(Fos) cited in "Dihydroartemisinin imposes positive and negative regulation on Treg and plasma cells via direct interaction and activation of c-Fos". Communications biology, 2023

Recombinant Human Keratin, type I cytoskeletal 18(KRT18) cited in "Calcitonin gene-related peptide ameliorates sepsis-induced intestinal injury by suppressing NLRP3 inflammasome activation". International immunopharmacology, 2023

Recombinant Human Protein Wnt-5a (WNT5A) cited in "ATBF1 is a potential diagnostic marker of histological grade and functions via WNT5A in breast cancer". BMC Cancer, 2022

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